Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

  • 中文名称：
    人D-乳酸脱氢酶(D-LDH)酶联免疫试剂盒
  • 货号：
    CSB-E11720h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LDHD ELISA Kit was designed for the quantitative measurement of Human LDHD protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 mIU/mL-2000 mIU/mL and the sensitivity is 7.8 mIU/mL.

  • 别名：
    D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
  • 缩写：
    LDHD
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 mIU/mL-2000 mIU/mL
  • 灵敏度：
    7.8 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %87-103
    1:2Average %91
    Range %86-103
    1:4Average %94
    Range %86-105
    1:8Average %91
    Range %84-100
  • 回收率：
    The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9791-106
    EDTA plasma (n=4)9686-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    20002.418 2.385 2.402 2.265
    10001.773 1.728 1.751 1.614
    5000.936 1.002 0.969 0.832
    2500.624 0.651 0.638 0.501
    1250.407 0.422 0.415 0.278
    62.50.282 0.287 0.285 0.148
    31.250.253 0.264 0.259 0.122
    00.135 0.139 0.137
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in D-lactate, but not L-lactate catabolic process.
  • 基因功能参考文献：
    1. The activity and expression of the mitochondrial D-lactate dehydrogenase, is higher in prostate cancer versus normal prostate cells. PMID: 23333299
    2. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
    3. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    FAD-binding oxidoreductase/transferase type 4 family
  • 组织特异性：
    Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney.
  • 数据库链接：

    HGNC: 19708

    OMIM: 607490

    KEGG: hsa:197257

    STRING: 9606.ENSP00000300051

    UniGene: Hs.380929