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  4. Rabbit Leptin,LEP ELISA Kit

Rabbit Leptin,LEP ELISA Kit

  • 中文名称：
    兔子瘦素(LEP)酶联免疫试剂盒
  • 货号：
    CSB-E06971Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rabbit LEP ELISA Kit was designed for the quantitative measurement of Rabbit LEP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 缩写：
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 91-99  
    1:200 Average % 93  
    Range % 88-97  
    1:400 Average % 85  
    Range % 80-89  
    1:800 Average % 90  
    Range % 84-96  
  • 回收率：
    The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 87 83-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.097 0.098 0.098    
    500 0.267 0.271 0.269    
    250 0.477 0.466 0.472    
    125 0.703 0.691 0.697    
    62.5 1.104 1.091 1.098    
    31.25 1.412 1.429 1.421    
    15.6 1.606 1.705 1.656    
    0 1.813 1.915 1.864    
  • 数据处理：
  • 货期：
    3-5 working days

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