Rat High density lipoprotein cholesterol, HDL-C ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Rat HDL-C ELISA Kit was designed for the quantitative measurement of Rat HDL-C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 ng/mL-6000 ng/mL and the sensitivity is 23.4 ng/mL.
缩写：

HDL-C
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 ng/mL-6000 ng/mL
灵敏度：

23.4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	87
	Range %	83-89
1:400	Average %	99
	Range %	95-102
1:800	Average %	90
	Range %	85-93
1:1600	Average %	99
	Range %	92-102

回收率：

The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	107	101-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.