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  4. Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

  • 中文名称：
    大鼠L-乳酸脱氢酶(L-LDH)酶联免疫试剂盒
  • 货号：
    CSB-E11324r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rat LDHA ELISA Kit is engineered for accurate measurement of rat LDHA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the LDHA content in the sample. The color intensity is positively correlated with LDHA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    LDHA is a cytosolic enzyme that catalyzes the conversion of pyruvate to lactate under anaerobic conditions. It is primarily found in skeletal muscle. LDHA is necessary to maintain glycolysis and ATP synthesis in the absence of sufficient oxygen by recycling NAD+ from NADH. LDHA also participates in the modulation of transcription by regulating the cellular redox state. During the differentiation of thymocytes, LDHA also functions as a molecular chaperone or as an association molecule. Even when oxygen is available, cancer cells employ LDHA to boost glycolysis, ATP, and lactate synthesis. Overexpression of LDHA has been associated with a number of additional unfavorable prognostic variables, including tumor hypoxia, angiogenesis, proliferation, and glucose uptake, as well as chemotherapy and radiotherapy resistance.

  • 别名：
    Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 mU/mL-2500 mU/mL
  • 灵敏度：
    9.77 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %80-97
    1:2Average %92
    Range %88-105
    1:4Average %97
    Range %92-104
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-100
    EDTA plasma (n=4)9085-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    25002.754 2.952 2.853 2.679
    12502.218 2.376 2.297 2.123
    6251.534 1.584 1.559 1.385
    312.51.022 1.039 1.031 0.857
    1560.587 0.624 0.606 0.432
    780.412 0.430 0.421 0.247
    390.286 0.279 0.283 0.109
    00.173 0.175 0.174 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 基因功能参考文献：
    1. LDHA is a direct target of miR-34a, which binds to the 3'-UTR region of LDHA mRNA in cardiomyocytes. PMID: 28894025
    2. role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells PMID: 28818664
    3. After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function. PMID: 27654895
    4. The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
    5. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration PMID: 24835193
    6. Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. PMID: 24086675
    7. lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
    8. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    LDH/MDH superfamily, LDH family
  • 数据库链接：