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Mouse Calcitonin,CT ELISA Kit

  • 中文名称:
    小鼠降钙素(CT)酶联免疫试剂盒
  • 货号:
    CSB-E05133m
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 别名:
    CALCA Calcitonin
  • 缩写:
    CALCA
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    12.5 pg/mL-800 pg/mL
  • 灵敏度:
    3.12 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 86  
    Range % 83-89  
    1:8 Average % 97  
    Range % 92-102  
  • 回收率:
    The recovery of mouse CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 82-93  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    800 2.026 2.068 2.047 1.945  
    400 1.412 1.465 1.439 1.337  
    200 0.908 0.935 0.922 0.820  
    100 0.568 0.534 0.551 0.449  
    50 0.346 0.326 0.336 0.234  
    25 0.269 0.268 0.269 0.167  
    12.5 0.189 0.198 0.194 0.092  
    0 0.101 0.103 0.102    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能:
    Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Calcitonin family
  • 数据库链接: