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  4. Human Ghrelin(GHRL) ELISA Kit

Human Ghrelin(GHRL) ELISA Kit

  • 中文名称：
    人饥饿激素(GHRL)酶联免疫试剂盒
  • 货号：
    CSB-E13398h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GHRL ELISA Kit was designed for the quantitative measurement of Human GHRL protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL.

  • 别名：
    Appetite regulating hormone ELISA Kit; Ghrelin 27 ELISA Kit; Ghrelin 28 ELISA Kit; Ghrelin and obestatin prepropeptide ELISA Kit; Ghrelin ELISA Kit; Ghrelin/obestatin prepropeptide ELISA Kit; ghrl ELISA Kit; GHRL_HUMAN ELISA Kit; Growth hormone releasing peptide ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; M46 protein ELISA Kit; Motilin related peptide ELISA Kit; Motilin-related peptide ELISA Kit; MTLRP ELISA Kit; Obestatin ELISA Kit; Protein M46 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.625 pg/mL-40 pg/mL
  • 灵敏度：
    0.156 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %86
    Range %80-92
    1:200Average %99
    Range %91-105
    1:400Average %101
    Range %92-110
    1:800Average %93
    Range %86-98
  • 回收率：
    The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
    EDTA plasma (n=4)9490-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    402.921 2.903 2.912 2.826
    202.405 2.348 2.383 2.297
    101.663 1.643 1.653 1.567
    51.053 0.989 1.021 0.935
    2.50.575 0.603 0.589 0.503
    1.250.322 0.319 0.321 0.235
    0.6250.214 0.229 0.222 0.136
    00.089 0.083 0.086 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GHRL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled GHRL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

I need Test Elisa for Human Acylated Ghrelin Test ....do you can help me...what is it?
and do you have this product?

A:
Thanks for your inquiry.
Yes,we have the requested kit available:
#CSB-E13398h,Human Ghrelin(GHRL) ELISA Kit.It is for acylated Ghrelin. Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.; Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • 基因功能参考文献：
    1. In this review we highlight the multifaceted nature of ghrelin and summarize its glucoregulatory action and discuss the pharmacological value of ghrelin pathway inhibition for the treatment of glucose intolerance and type 2 diabetes. PMID: 29412824
    2. This review examines the biochemical properties of the obestatin peptide (its structure, sequence, stability and distribution) and the candidate receptors through which it may act. It provides a balanced examination of the reported pancreatic and extrapancreatic actions of obestatin and evaluates its potential relevance with respect to diabetes therapy PMID: 29412827
    3. Expression of ghrelin and GHSR1a was studied in human pituitary adenomas. PMID: 28377557
    4. co-administration of Ghr and GH is a promising therapeutic tool for reversing immunosuppression caused by sepsis in the geriatric population. PMID: 28115288
    5. The aim of this study was to determine serum ghrelin and leptin levels in obese and lean Saudi women with Polycystic ovary syndrome and to investigate their relationship to the metabolic profiles in these women. PMID: 30131073
    6. A better understanding of ghrelin in depression could potentially help to optimize future therapeutic --{REVIEW} PMID: 29395244
    7. After Roux-en-Y Gastric bypass, over expression of Ghrelin gene occurs only in the excluded stomach with no correlation to T2 diabetes remission. PMID: 29307107
    8. our data indicate that the splice variant In1-ghrelin could play relevant functional roles in the regulation of breast cancer development and progression PMID: 29272342
    9. down-regulation of ghrelin receptors in substantia nigra pars compacta-dopaminergic (DA) neurons induced the initial dysfunction of DA neurons, leading to extrapyramidal disorder under Parkinson's disease. PMID: 29458391
    10. Data suggest that expression of mRNA for ghrelin and VEGFA are up-regulated in endometrium of women with recurrent miscarriage; thus, ghrelin and VEGFA may play roles in pathogenesis of recurrent miscarriage. These case-control studies were conducted with endometrial tissue obtained during secretory phase of menstrual cycle. (VEGFA = vascular endothelial growth factor A) PMID: 29221937
    11. Serum level of obestatin was significantly lower in the non-diabetic obese patients.Serum obestatin level strongly correlates with lipoprotein subfractions in non-diabetic obese patients. PMID: 29506551
    12. H. pylori infection and severity of gastric corpus pathology are associated with lower serum ghrelin. PMID: 29391762
    13. Data confirm that cord blood levels of ghrelin, leptin, and insulin of term newborns correlate with anthropometric parameters at birth (birth weight, head circumference, etc.). PMID: 29320365
    14. Concentrations of ghrelin were significantly lower in breast milk than in blood plasma from women that carried to term or gave birth prematurely. PMID: 29375043
    15. Individuals with higher levels of fasting ghrelin are more sensitive to reward, but less sensitive to PMID: 29136100
    16. low Plasma ghrelin expression is associated with metabolic syndrome. PMID: 28539201
    17. H. pylori infection decreased serum concentrations of leptin and obestatin in Mexican schoolchildren, suggesting alterations in regulating appetite and energy homeostasis. PMID: 28422951
    18. findings indicate that neither -604G/A and nor -501A/C polymorphisms of ghrelin gene are associated with PCOS, but suggest a relation between the presence of polymorphic allele of -501A/C polymorphism and LDL-C level in a sample of Iranian women. PMID: 29230615
    19. he 72Met allele of the preproghrelin Leu72Met polymorphism may be associated with rehabilitation of depression in male Chinese Han adolescents after the natural disaster. PMID: 28570394
    20. ghrelin effectively suppressed TNF-alpha-induced inflammatory factors' (including ICAM-1, VCAM-1, MCP-1, and IL-1beta) expression through inhibiting AMPK phosphorylation and p65 expression both in HUVEC and THP-1. PMID: 28653238
    21. The results showed that pretreatment with ghrelin reduced H2O2-induced cellular apoptosis and ROS accumulation, increased the expression levels of SOD and CAT, and decreased the expression level of MDA. PMID: 29129986
    22. Medium chain triglyceride nutritional supplementation increased the amount of activated ghrelin and NPY in anorexia nervosa patients. PMID: 28361739
    23. The establishment of the exact correlation between ghrelin, appetite and obesity could be vital for the fight against obesity. PMID: 29102924
    24. Studied expression of In1-ghrelin splice variant in prostate cancer and its possible role in tumorigenesis and disease progression. PMID: 28851363
    25. These results indicate that ghrelin promotes A549cell proliferation via GHSR-dependent PI3K/Akt/mTOR/P70S6K and ERK signaling pathways. PMID: 29524402
    26. findings are the first to associate methylation levels in blood with brain activity in obesity-related regions, and further support previous findings between ghrelin, brain activity and genetic differences PMID: 28194012
    27. The results of this study showed that higher serum levels of obestatin were associated with macro albuminuria suggesting that obestatin may have a role in underlying pathogenic mechanisms that leads to diabetic nephropathy. PMID: 28091434
    28. The level of serum ghrelin might be a biomarker of executive function and become a strong predictor of executive function impairment in patients with type 2 diabetes mellitus. PMID: 27689345
    29. Study found three gene variants (CLOCK-rs4864548, PEMT-rs936108, and GHRELIN-rs696217) that exhibited uncorrected gene-by-sleep duration interactions in relation to BMI z-scores in a cohort of New Zealand European children. However, no interactions were identified in percentage body fat differences. Notably, these interactions are evident without detectable effects on sleep duration. PMID: 28899534
    30. In short, thin children, despite elevated ghrelin production, the low IGF-I concentration is observed, probably due to undernutrition and worse IGF-I formation. In short, normal-weight children and in short, obese ones, ghrelin and IGF-I production is normal, and it seems that mechanisms responsible for their short stature are other than low IGF-I. PMID: 27557428
    31. findings suggest that the Leu72Leu genotype may lead to increased risk of Alcohol Use Disorder possibly via mechanisms involving a lower response to alcohol resulting in excessive alcohol consumption. PMID: 28481975
    32. Ghrelin is involved in appetite-regulating pathways during depression. PMID: 24671339
    33. Data suggest that subjects with anorexia nervosa display broad spectrum of physical activity (2479-26,047 steps/day) which shows a negative correlation with plasma kisspeptin levels and a positive association with plasma ghrelin levels. PMID: 27693487
    34. ghrelin was able to activate the proteasome in neural cells playing also a role in the interplay between the ubiquitin-proteasome system and autophagy. PMID: 26033219
    35. The G/G genotype of the A-604G SNP in the GHRL gene is associated with altered serum ghrelin levels and obesity. PMID: 28597412
    36. Ghrelin SNPs rs26802, rs10490816, and rs696217 were not associated with risk for metabolic syndrome in Chinese Han population. PMID: 28164505
    37. Carrying a G to T substitution in rs696217 preproghrelin gene seems to mark a successful weight loss outcome after gastric bypass in morbidly obese patients PMID: 27681093
    38. Low plasma ghrelin level is associated with mild cognitive impairment in type 2 diabetic patients. PMID: 28146431
    39. There might be a strong correlation between FGF-23 and ghrelin levels irrespective of the stage of chronic kidney disease and the dialysis modality PMID: 26125281
    40. Acylated ghrelin increases in obese individuals pre- and 30, 60, 90 and 120 minutes post prandial. PMID: 28625043
    41. Hemodialysis improves upper GI symptoms and gastric slow waves in CKD patients. An increase in ghrelin and a decrease in GLP-1 might be involved in the HD-induced improvement in gastric slow waves. PMID: 28566304
    42. In the obese polycystic ovary syndrome group, anti-mullerian hormone was associated with ghrelin levels independent of age, insulin, and total testosterone. There was no association between total ghrelin and anti-mullerian hormone levels in non-obese women with polycystic ovary syndrome, non-obese controls, or obese controls. PMID: 28004236
    43. Circulating obestatin levels were not different between lean and obese participants. PMID: 28174289
    44. Studies suggest that metformin treatment was not associated with a decrease in blood leptin levels, or increases in blood ghrelin levels in patients with type 2 diabetes mellitus (T2DM). PMID: 27380451
    45. Ghrelin was significantly higher and the obestatin/ghrelin ratio was significantly lower in celiac disease (CD) patients compared with both diarrhea-predominant irritable bowel syndrome (IBS-d) and healthy controls (HC). Significant differences were found in the Leu72Met polymorphism among groups, with the reduction of the GT genotype and the T allele in both CD and IBS-d patients compared with HC. PMID: 27750262
    46. Synovial fluid ghrelin levels demonstrated an independent and negative association with meniscus injury, cartilage damage, and clinical severity in patients with anterior cruciate ligament deficiency. PMID: 28147215
    47. Based on the obtained results, it was proposed that ghrelin may be considered as playing a role in the etiopathogenesis of hyperemesis gravidarum that may result in disruption of the relationship between nesfatin-1 and ghrelin. PMID: 27235705
    48. In hemodialysis patients, plasma ghrelin level was associated with the endoscopic and serological severity of atrophy related to H. pylori infection. PMID: 28058025
    49. studies indicate that pharmacological targeting of the endogenous ghrelin system is a valuable approach to treat metabolic complications. PMID: 28398233
    50. Patients with intestinal metaplasia, a known precursor of gastric cancer, had significantly less plasma ghrelin levels and ghrelin/obestatin (G/O) ratio than those without intestinal metaplasia. PMID: 28419119

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Motilin family
  • 组织特异性：
    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • 数据库链接：

    HGNC: 18129

    OMIM: 605353

    KEGG: hsa:51738

    STRING: 9606.ENSP00000335074

    UniGene: Hs.590080