CD247

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody (CSB-MA878942A1m, antigen from E.coli), the EC₅₀ of human PD-L1 protein is 1.252-1.653 ng/mL

The purity of CD274 was greater than 90% as determined by SEC-HPLC.

Western Blot
Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate.All lanes: CD274 Antibody at 1:1000.Secondary.Goat polyclonal to rabbit IgG at 1/50000 dilution,Predicted band size: 34, 21, 21 kDa,Observed band size: 45 kDa

IHC image of CSB-RA977797A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.