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  4. Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain (Cd247), partial

Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain (Cd247), partial

  • 中文名称：
    Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain(Cd247),partial,Yeast
  • 货号：
    CSB-YP004904MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain(Cd247),partial,Yeast
  • 货号：
    CSB-EP004904MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain(Cd247),partial,Yeast
  • 货号：
    CSB-EP004904MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain(Cd247),partial,Yeast
  • 货号：
    CSB-BP004904MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse T-cell surface glycoprotein CD3 zeta chain(Cd247),partial,Yeast
  • 货号：
    CSB-MP004904MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cd247; Cd3z; TcrzT-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 表达区域：
    52-164
  • 氨基酸序列
    RAKFSRSAETAANLQDPNQLYNELNLGRREEYDVLEKKRARDPEMGGKQQRRRNPQEGVYNALQKDKMAEAYSEIGTKGERRRGKGHDGLYQGLSTATKDTYDALHMQTLAPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN).
  • 基因功能参考文献：
    1. ITAM sequence diversity is required for optimal TCR signal transduction and subsequent T cell maturation PMID: 28733484
    2. Comprehensive Survey of miRNA-mRNA Interactions Reveals That Ccr7 and Cd247 (CD3 zeta) are Posttranscriptionally Controlled in Pancreas Infiltrating T Lymphocytes of Non-Obese Diabetic (NOD) Mice PMID: 26606254
    3. There is an early (within seconds) interaction between CD3zeta and the coreceptor CD8 that is independent of the binding of CD8 to MHC. PMID: 25427562
    4. The resting TCR localized in the disordered domain of giant plasma membrane (PM) vesicles (GPMVs) and PM spheres (PMSs) and single and nanoclustered TCRs are found in the high-density fractions in sucrose gradients. PMID: 25535948
    5. When Ly108 is engaged in trans during cell-cell interactions, Ly108-CD3zeta interactions are promoted in a manner that uniquely depends on Ly108 TM domain, leading to more efficient CD3zeta dephosphorylation. PMID: 25217164
    6. Two transcription factor binding sites and a long non-coding RNA are identified within the Cd247 gene. PMID: 24797614
    7. Zfat-deficiency results in a loss of CD3zeta phosphorylation with dysregulation of ERK and Egr activities leading to impaired positive selection. PMID: 24098453
    8. The results of this study supported the model that the procognitive function of CD3zeta may be mediated through its involvement in the NMDAR downstream signaling pathway leading to CaMKII-dependent LTP induction. PMID: 24259588
    9. T cell CD3zeta deficiency enables multiorgan tissue inflammation. PMID: 23980209
    10. Tyrosine phosphorylation of the TCR-zeta cytoplasmic domain regulates its association with the plasma membrane PMID: 22084078
    11. The data of this study suggested that CD3zeta mediates the ZAP-70/Syk kinase activation triggered by ephrinA-activated pathway to regulate early neuronal morphogenesis. PMID: 21895656
    12. Data show that TCRzeta phosphorylation signal pathways were affected in CD3gamma(-/-) primary and HVS-transformed T cells. PMID: 21764047
    13. functional role for CD3 zeta basic-rich stretch -phosphoinositide interactions in supporting T cell activation PMID: 21543646
    14. Results suggest that RhoH regulates TCR signaling via recruitment of ZAP-70 and Lck to CD3zeta in the immunological synapse. PMID: 21103055
    15. an intracellular pool of phosphorylated CD3zeta may help to sustain TcR/CD3 signaling after the receptor internalization. PMID: 21135224
    16. The allele of the Cd3zeta gene expressed in NOD and DBA/2 mouse strains confers lower levels of T cell activation compared with the allele expressed by C57BL/6, BALB/c, and C3H/HeJ mice. PMID: 20400699
    17. GRAIL, by mediating TCR-CD3 degradation, regulates naive T cell tolerance induction and Treg cell function PMID: 20493730
    18. a full complement of functional CD3 zeta immunoreceptor tyrosine-based activation motifs is required for effective iNKT cell development. PMID: 20483726
    19. We show that CD3zeta is expressed in retinal ganglion cells (RGCs). CD3zeta-deficient mice have reduced RGC dendritic motility, an increase in RGC dendritic density, and a selective defect of glutamate-receptor-mediated synaptic activity in the retina. PMID: 20188655
    20. Data show that In quiescent T cells, both TCR and Lat existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. PMID: 20010844
    21. experimental, computational and evolutionary approaches predicts the presence of a tetrameric form for CD3-zeta PMID: 11851345
    22. Signaling through the TCRzeta chain alone is insufficient to generate downstream events for full T-cell activation or thymocyte selection. Additional CD3 components must be required for a functional response in primary thymocytes and peripheral T cells. PMID: 12088411
    23. the zeta-chain stabilizes TCR residency on the cell surface, and functions to maintain cell surface receptor expression by sterically blocking internalization sequences in other TCR components PMID: 12444133
    24. Down-regulation of the invariant TCRzeta chain has been documented in a wide variety of chronic inflammatory and infectious diseases, and is thought to contribute to the paradoxical immune suppression observed in these diseases PMID: 14745016
    25. In the absence of the T cell receptor zeta-chain, CD4+ T cells develop as polarized interferon-gamma-producing cells that bear a memory cell-like phenotype. PMID: 15661872
    26. CREM alpha binds to the TCR zeta promoter and repress its activity. PMID: 16237091
    27. Constitutively phosphorylated CD3zeta (p21) was present in thymocytes that developed under nonselecting peptide-MHC conditions. PMID: 17371967
    28. These results suggest that BTLA functions to regulate T-cell signaling by controlling the phosphorylated form of TCRzeta accumulation in the lipid raft. PMID: 17607320
    29. We believe that these 'photo-switchable' conjugates could be used to markedly improve the targeting of the immune response to folate receptor (FR) expressing ovarian and breast cancers. PMID: 18068664
    30. In conclusion, CS1 and CS2 may be responsible for the regulation of zeta and TCR/CD3 through the stability of zeta mRNA in SLE T cells. PMID: 18177736
    31. The variation in TCRzeta expression was associated with polymorphisms in the CD3Z 3'-untranslated region (UTR) in SLE patients and healthy controls. PMID: 18178846
    32. Defect in CD3-zeta signaling mediates T cell hypo-responsiveness in mesenteric lymph nodes. PMID: 18619676
    33. A novel method using engineered cells (InsCD3-zeta) to target insulin-specific CD8 T cells. PMID: 19120267
    34. Cdc42 activity is impaired in beta1 integrin-deficient T cells that form conjugates with antigen-presenting cells but is partially restored in the context of an antigen-specific synapse. PMID: 19307303
    35. 9-bp deletion in the 3'-UT region of the CD3zeta gene is associated with cutaneous angiosarcoma and gastric metastases. PMID: 19775377

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    CD3Z/FCER1G family
  • 组织特异性：
    CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs). Expressed also in retinal ganglion cells.
  • 数据库链接：

    KEGG: mmu:12503

    STRING: 10090.ENSMUSP00000027849

    UniGene: Mm.217308