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ENPP3

外核苷酸焦磷酸酶/磷酸二酯酶家族成员3是一种由ENPP3基因编码的酶。Hiroaki在2013年的实验中对N2a细胞进行研究,纯化了存在于GnT-IX中的一种内源性抑制因子,发现其与外核苷酸焦磷酸酶/磷酸二酯酶3 (ENPP3)相同,这一结果通过质谱分析以及培养细胞中ENPP3的敲除和过表达得到了证明。动力学分析显示,ENPP3导致的GnT-IX抑制机制是ENPP3介导的核苷酸糖供体底物UDP-GlcNAc水解,从而生成UMP(一种强效竞争性GnT-IX抑制剂)。实际上,当对细胞内的ENPP3敲除,细胞内核苷酸糖水平显著升高,并显示出细胞总糖基化特征的变化。除了糖基转移酶的伴侣或其他已知调节剂外,ENPP3介导的核苷酸糖水解将对糖基转移酶活性产生也会产生广泛和显著的影响,并负责改变细胞总糖基化特征和调节细胞功能。

热销产品

Recombinant Macaca fascicularis Ectonucleotide pyrophosphatase/phosphodiesterase 3(ENPP3),partial (Active) (CSB-MP4278MOV)

验证数据

SDS

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELISA

Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 at 2 μg/ml can bind Anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 3.313-4.724 ng/mL.

Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3(ENPP3),partial (Active)(CSB-MP007681HU)

验证数据

SDS

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELISA

Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/ml can bind anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 2.151-2.492 ng/mL.

ENPP3 Recombinant Monoclonal Antibody CSB-RA007681MA1HU

验证数据

ELISA
CSB-RA007681MA1HU ELISA

The Binding Activity of Macaca fascicularis ENPP3 with Anti-ENPP3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 (CSB-MP4278MOV) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC50 is 3.313-4.724 ng/mL.

ELISA
CSB-RA007681MA1HU ELISA

The Binding Activity of Huamn ENPP3 with Anti- ENPP3 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3(CSB-MP007681HU) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC50 is 2.151-2.492 ng/mL

FC
CSB-RA007681MA1HU FC

Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T stable cells (red line) were stained with anti-ENPP3 recombinant antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

HEK293T/Human ENPP3 Stable Cell Line CSB-SC007681HU

验证数据

FC
CSB-SC007681HU FC

Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T Stable cells (red line) were stained with anti-ENPP3 antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

ENPP3 Antibodies

ENPP3 for Homo sapiens (Human)

ENPP3 Proteins

ENPP3 Proteins for Rattus norvegicus (Rat)

ENPP3 Proteins for Bos taurus (Bovine)

ENPP3 Proteins for Homo sapiens (Human)

ENPP3 Proteins for Pongo abelii (Sumatran orangutan)

ENPP3 Proteins for Mus musculus (Mouse)

ENPP3 ELISA Kit

ENPP3 ELISA Kit for Homo sapiens (Human)