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  4. Canine acetylcholine receptor antibody,AChRab ELISA Kit

Canine acetylcholine receptor antibody,AChRab ELISA Kit

  • 中文名称：
    犬乙酰胆碱受体抗体(AChRab)酶联免疫试剂盒
  • 货号：
    CSB-E08375c
  • 规格：
    96T/48T
  • 价格：
    ￥4600/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog AChR Ab ELISA Kit was designed for the quantitative measurement of Dog AChR Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.75 ng/mL-240 ng/mL and the sensitivity is 0.94 ng/mL.

  • 缩写：
    AChR Ab
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.75 ng/mL-240 ng/mL
  • 灵敏度：
    0.94 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine AChRab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-102  
    1:2 Average % 95  
    Range % 84-100  
    1:4 Average % 96  
    Range % 90-103  
    1:8 Average % 94  
    Range % 82-101  
  • 回收率：
    The recovery of canine AChRab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 92 87-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    240 2.954 2.918 2.936 2.832  
    120 2.512 2.631 2.572 2.468  
    60 1.852 1.873 1.863 1.759  
    30 1.034 0.996 1.015 0.911  
    15 0.472 0.494 0.483 0.379  
    7.5 0.261 0.276 0.269 0.165  
    3.75 0.155 0.161 0.158 0.054  
    0 0.102 0.106 0.104 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I want to use this kit to test the possibility of myasthenia gravis.

A:
Thanks for your inquiry. It is ok to test possibility of myasthenia gravis but we did not ever validate it. Pls follow your actual test result.