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  4. Chicken Tumor necrosis factor α,TNF-α ELISA KIT

Chicken Tumor necrosis factor α,TNF-α ELISA KIT

  • 中文名称：
    鸡肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号：
    CSB-E11231Ch
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        TNF-α（Tumor Necrosis Factor-alpha，肿瘤坏死因子-alpha）是一种由多种免疫细胞产生的细胞因子，具有多种生物学活性，包括促炎和抗炎作用。TNF-α主要由巨噬细胞、淋巴细胞和树突细胞等细胞合成，并参与了炎症和免疫反应等生理过程。在炎症反应中，TNF-α能够引起局部炎症反应，增强炎症细胞的活性，并参与组织修复和免疫细胞的调节。然而，过度产生的TNF-α也与多种疾病的发生和发展相关，如类风湿关节炎、炎症性肠病和心血管疾病等。
        j9九游会登录入口首页生物所提供的Chicken Tumor necrosis factor α,TNF-α ELISA KIT属于ELISA检测试剂盒，采用竞争法定量检测鸡血清、血浆、组织匀浆样本中的TNF-α，其灵敏度为0.27 pg/mL，检测范围为0.27 pg/mL-200 pg/mL。
     
  • 别名：
    DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
  • 缩写：
    TNF-α
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.27 pg/mL-200 pg/mL
  • 灵敏度：
    0.27 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-96  
    1:2 Average % 100  
    Range % 95-105  
    1:4 Average % 87  
    Range % 82-91  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 93-108  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.173 0.170 0.172    
    66.7 0.259 0.251 0.255    
    22.2 0.347 0.367 0.357    
    7.41 0.452 0.488 0.470    
    2.47 0.611 0.633 0.622    
    0.82 0.809 0.855 0.832    
    0.27 1.033 1.018 1.026    
    0 1.748 1.707 1.728    
  • 数据处理：
  • 货期：
    3-5 working days

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靶点详情

  • 最新研究进展：
    TNF-α（肿瘤坏死因子-α）是一种促炎症蛋白质，它在多种疾病中扮演重要角色。最新研究表明，TNF-α可能参与COVID-19的发病机制，因为这种病毒通过激活NF-κB途径来增加TNF-α的表达，从而引发炎症反应和危及生命的并发症。此外，TNF-α也参与了许多自身免疫疾病的发生和发展过程，例如风湿性关节炎和肠道炎症性疾病。