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Fish Complement 3,C3 ELISA Kit

  • 中文名称：
    鱼补体蛋白3(C3)酶联免疫试剂盒
  • 货号：
    CSB-E09727s
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Fish Complement 3 (C3) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 12.5 μg/mL-500 μg/mL and the sensitivity is 12.5 μg/mL.

  • 缩写：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma
  • 检测范围：
    12.5 μg/mL-500 μg/mL
  • 灵敏度：
    12.5 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-100  
    1:2 Average % 96  
    Range % 90-108  
    1:4 Average % 95  
    Range % 91-98  
    1:8 Average % 95  
    Range % 91-100  
  • 回收率：
    The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 95 91-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average 　  
    500 0.176 0.174 0.175 　  
    250 0.381 0.393 0.387 　  
    115 0.557 0.569 0.563 　  
    37.5 0.894 0.901 0.898 　  
    12.5 1.481 1.498 1.490 　  
    0 2.417 2.542 2.480 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Serum
    Sample species: Fish
    Review: Instructions that come with product are easy to understand and easy to use. Every part of the kit is clearly labelled and easily identifiable. Standards come prediluted which makes them even easier to use. In this experiment we decided to test Fish C3 levels in fish fed a basal feed and fish fed a specialized feed containing a IMP We found there was an increased level of C3 in fish fed the IMP.