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  4. Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

  • 中文名称：
    人Ⅲ型胶原(Col Ⅲ)酶联免疫试剂盒
  • 货号：
    CSB-E04799h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human Col Ⅲ ELISA Kit was designed for the quantitative measurement of Human Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL .

  • 别名：
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 缩写：
    Col Ⅲ
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-96  
    1:2 Average % 102  
    Range % 97-105  
    1:4 Average % 92  
    Range % 85-98  
    1:8 Average % 97  
    Range % 91-102  
  • 回收率：
    The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 80-94  
    EDTA plasma (n=4) 97 92-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    800 2.850 2.963 2.907 2.778  
    400 2.255 2.203 2.229 2.100  
    200 1.279 1.256 1.268 1.139  
    100 0.659 0.655 0.657 0.528  
    50 0.438 0.475 0.457 0.328  
    25 0.256 0.242 0.249 0.120  
    12.5 0.184 0.172 0.178 0.049  
    0 0.126 0.132 0.129 　  
  • 数据处理：
  • 货期：
    3-5 working days

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