Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

  • 中文名称：
    大鼠Ⅲ型胶原(Col Ⅲ)酶联免疫试剂盒
  • 货号：
    CSB-E07924r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Col Ⅲ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 别名：
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 缩写：
    Col Ⅲ
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 98  
    Range % 92-104  
    1:4 Average % 95  
    Range % 92-98  
    1:8 Average % 86  
    Range % 80-94  
  • 回收率：
    The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 90 85-95  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.585 2.532 2.559 2.415  
    5 1.795 1.719 1.757 1.613  
    2.5 1.083 1.021 1.052 0.908  
    1.25 0.657 0.705 0.681 0.537  
    0.625 0.418 0.450 0.434 0.290  
    0.312 0.299 0.314 0.307 0.163  
    0.156 0.242 0.229 0.236 0.092  
    0 0.146 0.141 0.144 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：大鼠

稀释比：1:50

产品评价： RAT collagen type3, elisa试剂盒检测大鼠血清，复孔的数据较稳定。试剂盒溶液给量充足。此外，还发现该试剂盒可以检测其他啮齿动物的血清样本，但OD值偏小。

By 杜老师