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  4. Human Endothelial lipase,EL ELISA Kit

Human Endothelial lipase,EL ELISA Kit

  • 中文名称：
    人内皮脂肪酶(EL)酶联免疫试剂盒
  • 货号：
    CSB-E08217h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human LIPG ELISA Kit was designed for the quantitative measurement of Human LIPG protein in serum, plasma, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    EDL ELISA Kit; EL ELISA Kit; Endothelial cell-derived lipase ELISA Kit; Endothelial lipase ELISA Kit; Endothelial lipase precursor ELISA Kit; Lipase endothelial ELISA Kit; LIPE_HUMAN ELISA Kit; LIPG ELISA Kit; Lipoprotein lipase H ELISA Kit; PRO719 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, urine
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %83-102
    1:2Average %90
    Range %85-102
    1:4Average %93
    Range %89-99
    1:8Average %94
    Range %87-108
  • 回收率：
    The recovery of human EL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9786-104
    EDTA plasma (n=4)9589-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.415 2.386 2.401 2.271
    201.702 1.734 1.718 1.588
    101.204 1.195 1.200 1.070
    50.730 0.711 0.721 0.591
    2.50.417 0.429 0.423 0.293
    1.250.296 0.285 0.291 0.161
    0.6250.234 0.250 0.242 0.112
    00.127 0.132 0.130
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Exerts both phospholipase and triglyceride lipase activities. More active as a phospholipase than a triglyceride lipase. Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins.
  • 基因功能参考文献：
    1. the LIPG polymorphism (584 C/T) - CAD correlation study in Turkish people living in Turkey. Results of this study suggest that the CC genotype may be a genetic risk factor for CAD and carrying T allele may be protective against CAD. PMID: 30150432
    2. We identified a missense Asn396Ser mutation (rs77960347) in the endothelial lipase (LIPG) gene, occurring with an allele frequency of 1% in the general population, which was significantly associated with depressive symptoms (P-value=5.2 x 10-08, beta=7.2). PMID: 27431295
    3. the EL 2237 A allele might be associated with an increased Apo A1 level in Coronary Artery Disease subjects. PMID: 27612170
    4. Increased hepatic expression of endothelial lipase attenuates cholesterol diet-induced hypercholesterolemia and protects against atherosclerosis. PMID: 28546217
    5. Endothelial lipase is upregulated in human umbilical vein endothelial cells by interleukin-6 partly via the p38 MAPK and p65 NF-kappaB signaling pathways. PMID: 27430252
    6. Endothelial lipase (EL) 2037T/C and 2237 G/A polymorphisms might not affect the lipid-owing effects of rosuvastatin in Chinese coronary artery disease patients PMID: 27600285
    7. Endothelial lipase protein expression was increased in skeletal muscle of middle-aged men with high oxygen consumption. PMID: 26447519
    8. LIPG Polymorphisms are associated with Hyperlipidemia. PMID: 27590083
    9. FoxA1, FoxA2, and LIPG control the uptake of extracellular lipids for breast cancer growth. PMID: 27045898
    10. results suggested that the SNP -384A/C in the LIPG gene may be associated with risk for coronary artery disease(CAD)and the LIPG gene may play a role in CAD in the Han Chinese PMID: 26124511
    11. Studied if a specific variant of the endothelial lipase gene was more specifically linked to the severity of diabetic retinopathy. PMID: 24852509
    12. Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels. PMID: 23109900
    13. EL-384A/C polymorphism was significantly associated with the ACS and lipids profile in an elderly Uygur population in Xinjiang. PMID: 25291260
    14. Results showed that the protein levels of endothelial lipase (EL), NF-kappaB p65, MAPK p38 protein levels, in addition to the proliferation of umbilical vein endothelial cells (HUVECs), were increased by angiotensin II (AngII). PMID: 25250890
    15. Genetic studies of patients with early onset of coronary artery disease (CAD) in the Chinese Han population show that the EL 584C/T variant is not always involved in the pathogenesis of early-onset CAD. PMID: 24634127
    16. The results of the present meta-analysis suggest that the carriers of EL 584 T allele have a higher HDL-C level in Caucasian populations. Whereas, it might not be a protective factor for CHD. PMID: 24886585
    17. Results indicate the spatial consensus catalytic triad "Ser-Asp-His", a characteristic motif in lipoprotein lipase, hepatic lipase, and endothelial lipase. PMID: 23991054
    18. EL activity was higher in metabolic syndrome and in obesity. It was negatively associated with high-density lipoprotein-cholesterol and apolipoprotein A-I in control and metabolic syndrome, respectively. PMID: 24458708
    19. T allele of LIPG -584C/T polymorphism might play a potential role in the susceptibility to atherogenesis in the Turkish population. PMID: 23673478
    20. Interleukin-6 stimulates the translocation of HDL through the endothelium, the first step in reverse cholesterol transport pathway, by enhancing EL expression. PMID: 24115033
    21. Complete loss-of-function mutation of LIPG had higher plasma high-density cholesterol levels compared with partial loss-of-function mutation carriers. PMID: 23243195
    22. the EL -384A/C gene polymorphism might be associated with ACS in Chinese Han population, suggesting that the variant might be involved in the pathogenesis of ACS. PMID: 22723003
    23. Urinary endothelial lipase is a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity. PMID: 23510199
    24. Endothelial lipase supplies cells with free fatty acids and HDL-derived lysophosphatidylcholine and lysophosphatidylethanolamine species resulting in increased cellular triglycerides and phosphatidylcholine (PC) content and decreased PC synthesis. PMID: 23075452
    25. EL expression modulates vascular remodeling as well as plasma HDL-C levels PMID: 22972429
    26. Starvation regulates endothelial lipase expression via SREBP-2. PMID: 23102786
    27. analysis of LIPG, CETP, and GALNT2 mutations in Caucasian families with extremely high HDL cholesterol PMID: 22952570
    28. study tested the hypothesis that placental EL expression is affected by obesity in pregnancy and gestational diabetes; metabolic inflammation with high leptin and increased TNF-alpha concentration at fetal-placental interface regulates placental EL expression PMID: 21852675
    29. Endothelial lipase plays a role in thyroid and adipocyte biology in addition to its well-known role in endothelial function and HDL metabolism. PMID: 22740344
    30. Common and rare genetic variants in CETP and LIPC, but not LIPG, were more commonly found in the Thai HALP group, which could potentially contribute to high high-density lipoprotein cholesterol phenotypes in this population. PMID: 22464213
    31. a reduction in nascent HDL formation may be partly responsible for reduced HDL-C during inflammation when both EL and SAA are known to be upregulated PMID: 21957202
    32. we found that a common nonfunctional coding variant associated with HDL-C (rs2000813) is in linkage disequilibrium with a 5' UTR variant (rs34474737) that decreases LIPG promoter activity PMID: 22174694
    33. Treatment of patients with coronary artery disease with lipid-modulation and/or antiplatelet drug may significantly decrease the expression of endothelial lipase. PMID: 21122200
    34. results suggest that the subjects with TT endothelial lipase genotype benefit more from alcohol consumption than the subjects with CT and CC genotypes in increasing serum high-density lipoprotein cholesterol and apolipoprotein (Apo) AI levels PMID: 21816559
    35. The c.584C>T EL polymorphism is associated with a higher risk of diabetic retinopathy that could be linked to modifications in HDL-cholesterol metabolism and blood pressure levels PMID: 21145773
    36. Endothelial lipase and EL-generated lysophosphatidylcholines promote endothelial IL-8 synthesis. PMID: 21130993
    37. Sulforaphane inhibits endothelial lipase expression via inhibition of NF-kappaB which may have a beneficial effect on HDL cholesterol levels PMID: 20688330
    38. The LIPG 584T allele is associated with increased serum HDL-C, TC and ApoB levels. PMID: 20923576
    39. common genetic variation in endothelial lipase (LIPG) does not appear to have a role in risk for coronary artery disease and deep venous thrombosis PMID: 20466371
    40. Statins can reduce EL expression in vitro and in vivo via inhibition of RhoA activity. The inhibition of EL expression in the vessel wall may contribute to the anti-atherogenic effects of statins. PMID: 20045866
    41. LPL and LIPG are reported in the human testis and in germ cell neoplasms. PMID: 19780863
    42. It seems that plasma endothelial lipase levels in individuals with atherosclerosis might be higher than that measured in healthy individuals. [review] PMID: 20621031
    43. Thus, this N-terminal variant results in reduced secretion of endothelial lipase, plausibly leading to increased HDL-C levels. PMID: 19411705
    44. EDL mediates both HDL binding and uptake, and the selective uptake of HDL-CE, independently of lipolysis and CLA-1. PMID: 12164779
    45. Endothelial lipase has a role in HDL metabolism [review] PMID: 12569156
    46. EL is a major determinant of HDL concentration in humans PMID: 12601178
    47. Expression of human endothelial lipase in mice results in dose-dependent increase in postheparin plasma phospholipase activity, catabolic rate of HDL-apolipoprotein, and uptake of apoA-I in both kidney and liver PMID: 14517167
    48. Hepatic expression of human EL in mice resulted in markedly decreased levels of VLDL/LDL cholesterol, phospholipid, and apoB accompanied by significantly increased LDL apolipoprotein and phospholipid catabolism. PMID: 15117821
    49. N-linked glycosylation has a role in the secretion and activity of endothelial lipase PMID: 15342690
    50. results suggest that endothelial lipase (EL) on the endothelial cell surface can promote monocyte adhesion to the vascular endothelium through the interaction with heparan sulfate proteoglycans PMID: 15485805

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not
  • 数据库链接：

    HGNC: 6623

    OMIM: 603684

    KEGG: hsa:9388

    STRING: 9606.ENSP00000261292

    UniGene: Hs.465102