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  4. Recombinant Human Endothelial lipase (LIPG)

Recombinant Human Endothelial lipase (LIPG)

  • 货号：
    CSB-YP897572HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP897572HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP897572HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP897572HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP897572HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EDL; EL; Endothelial cell-derived lipase; Endothelial lipase; Endothelial lipase precursor ; Lipase endothelial ; LIPE_HUMAN; LIPG; Lipoprotein lipase H; PRO719
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    21-500
  • 氨基酸序列
    SPVPFGPEGR LEDKLHKPKA TQTEVKPSVR FNLRTSKDPE HEGCYLSVGH SQPLEDCSFN MTAKTFFIIH GWTMSGIFEN WLHKLVSALH TREKDANVVV VDWLPLAHQL YTDAVNNTRV VGHSIARMLD WLQEKDDFSL GNVHLIGYSL GAHVAGYAGN FVKGTVGRIT GLDPAGPMFE GADIHKRLSP DDADFVDVLH TYTRSFGLSI GIQMPVGHID IYPNGGDFQP GCGLNDVLGS IAYGTITEVV KCEHERAVHL FVDSLVNQDK PSFAFQCTDS NRFKKGICLS CRKNRCNSIG YNAKKMRNKR NSKMYLKTRA GMPFRVYHYQ MKIHVFSYKN MGEIEPTFYV TLYGTNADSQ TLPLEIVERI EQNATNTFLV YTEEDLGDLL KIQLTWEGAS QSWYNLWKEF RSYLSQPRNP GRELNIRRIR VKSGETQRKL TFCTEDPENT SISPGRELWF RKCRDGWRMK NETSPTVELP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exerts both phospholipase and triglyceride lipase activities. More active as a phospholipase than a triglyceride lipase. Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins.
  • 基因功能参考文献：
    1. the LIPG polymorphism (584 C/T) - CAD correlation study in Turkish people living in Turkey. Results of this study suggest that the CC genotype may be a genetic risk factor for CAD and carrying T allele may be protective against CAD. PMID: 30150432
    2. We identified a missense Asn396Ser mutation (rs77960347) in the endothelial lipase (LIPG) gene, occurring with an allele frequency of 1% in the general population, which was significantly associated with depressive symptoms (P-value=5.2 x 10-08, beta=7.2). PMID: 27431295
    3. the EL 2237 A allele might be associated with an increased Apo A1 level in Coronary Artery Disease subjects. PMID: 27612170
    4. Increased hepatic expression of endothelial lipase attenuates cholesterol diet-induced hypercholesterolemia and protects against atherosclerosis. PMID: 28546217
    5. Endothelial lipase is upregulated in human umbilical vein endothelial cells by interleukin-6 partly via the p38 MAPK and p65 NF-kappaB signaling pathways. PMID: 27430252
    6. Endothelial lipase (EL) 2037T/C and 2237 G/A polymorphisms might not affect the lipid-owing effects of rosuvastatin in Chinese coronary artery disease patients PMID: 27600285
    7. Endothelial lipase protein expression was increased in skeletal muscle of middle-aged men with high oxygen consumption. PMID: 26447519
    8. LIPG Polymorphisms are associated with Hyperlipidemia. PMID: 27590083
    9. FoxA1, FoxA2, and LIPG control the uptake of extracellular lipids for breast cancer growth. PMID: 27045898
    10. results suggested that the SNP -384A/C in the LIPG gene may be associated with risk for coronary artery disease(CAD)and the LIPG gene may play a role in CAD in the Han Chinese PMID: 26124511
    11. Studied if a specific variant of the endothelial lipase gene was more specifically linked to the severity of diabetic retinopathy. PMID: 24852509
    12. Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels. PMID: 23109900
    13. EL-384A/C polymorphism was significantly associated with the ACS and lipids profile in an elderly Uygur population in Xinjiang. PMID: 25291260
    14. Results showed that the protein levels of endothelial lipase (EL), NF-kappaB p65, MAPK p38 protein levels, in addition to the proliferation of umbilical vein endothelial cells (HUVECs), were increased by angiotensin II (AngII). PMID: 25250890
    15. Genetic studies of patients with early onset of coronary artery disease (CAD) in the Chinese Han population show that the EL 584C/T variant is not always involved in the pathogenesis of early-onset CAD. PMID: 24634127
    16. The results of the present meta-analysis suggest that the carriers of EL 584 T allele have a higher HDL-C level in Caucasian populations. Whereas, it might not be a protective factor for CHD. PMID: 24886585
    17. Results indicate the spatial consensus catalytic triad "Ser-Asp-His", a characteristic motif in lipoprotein lipase, hepatic lipase, and endothelial lipase. PMID: 23991054
    18. EL activity was higher in metabolic syndrome and in obesity. It was negatively associated with high-density lipoprotein-cholesterol and apolipoprotein A-I in control and metabolic syndrome, respectively. PMID: 24458708
    19. T allele of LIPG -584C/T polymorphism might play a potential role in the susceptibility to atherogenesis in the Turkish population. PMID: 23673478
    20. Interleukin-6 stimulates the translocation of HDL through the endothelium, the first step in reverse cholesterol transport pathway, by enhancing EL expression. PMID: 24115033
    21. Complete loss-of-function mutation of LIPG had higher plasma high-density cholesterol levels compared with partial loss-of-function mutation carriers. PMID: 23243195
    22. the EL -384A/C gene polymorphism might be associated with ACS in Chinese Han population, suggesting that the variant might be involved in the pathogenesis of ACS. PMID: 22723003
    23. Urinary endothelial lipase is a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity. PMID: 23510199
    24. Endothelial lipase supplies cells with free fatty acids and HDL-derived lysophosphatidylcholine and lysophosphatidylethanolamine species resulting in increased cellular triglycerides and phosphatidylcholine (PC) content and decreased PC synthesis. PMID: 23075452
    25. EL expression modulates vascular remodeling as well as plasma HDL-C levels PMID: 22972429
    26. Starvation regulates endothelial lipase expression via SREBP-2. PMID: 23102786
    27. analysis of LIPG, CETP, and GALNT2 mutations in Caucasian families with extremely high HDL cholesterol PMID: 22952570
    28. study tested the hypothesis that placental EL expression is affected by obesity in pregnancy and gestational diabetes; metabolic inflammation with high leptin and increased TNF-alpha concentration at fetal-placental interface regulates placental EL expression PMID: 21852675
    29. Endothelial lipase plays a role in thyroid and adipocyte biology in addition to its well-known role in endothelial function and HDL metabolism. PMID: 22740344
    30. Common and rare genetic variants in CETP and LIPC, but not LIPG, were more commonly found in the Thai HALP group, which could potentially contribute to high high-density lipoprotein cholesterol phenotypes in this population. PMID: 22464213
    31. a reduction in nascent HDL formation may be partly responsible for reduced HDL-C during inflammation when both EL and SAA are known to be upregulated PMID: 21957202
    32. we found that a common nonfunctional coding variant associated with HDL-C (rs2000813) is in linkage disequilibrium with a 5' UTR variant (rs34474737) that decreases LIPG promoter activity PMID: 22174694
    33. Treatment of patients with coronary artery disease with lipid-modulation and/or antiplatelet drug may significantly decrease the expression of endothelial lipase. PMID: 21122200
    34. results suggest that the subjects with TT endothelial lipase genotype benefit more from alcohol consumption than the subjects with CT and CC genotypes in increasing serum high-density lipoprotein cholesterol and apolipoprotein (Apo) AI levels PMID: 21816559
    35. The c.584C>T EL polymorphism is associated with a higher risk of diabetic retinopathy that could be linked to modifications in HDL-cholesterol metabolism and blood pressure levels PMID: 21145773
    36. Endothelial lipase and EL-generated lysophosphatidylcholines promote endothelial IL-8 synthesis. PMID: 21130993
    37. Sulforaphane inhibits endothelial lipase expression via inhibition of NF-kappaB which may have a beneficial effect on HDL cholesterol levels PMID: 20688330
    38. The LIPG 584T allele is associated with increased serum HDL-C, TC and ApoB levels. PMID: 20923576
    39. common genetic variation in endothelial lipase (LIPG) does not appear to have a role in risk for coronary artery disease and deep venous thrombosis PMID: 20466371
    40. Statins can reduce EL expression in vitro and in vivo via inhibition of RhoA activity. The inhibition of EL expression in the vessel wall may contribute to the anti-atherogenic effects of statins. PMID: 20045866
    41. LPL and LIPG are reported in the human testis and in germ cell neoplasms. PMID: 19780863
    42. It seems that plasma endothelial lipase levels in individuals with atherosclerosis might be higher than that measured in healthy individuals. [review] PMID: 20621031
    43. Thus, this N-terminal variant results in reduced secretion of endothelial lipase, plausibly leading to increased HDL-C levels. PMID: 19411705
    44. EDL mediates both HDL binding and uptake, and the selective uptake of HDL-CE, independently of lipolysis and CLA-1. PMID: 12164779
    45. Endothelial lipase has a role in HDL metabolism [review] PMID: 12569156
    46. EL is a major determinant of HDL concentration in humans PMID: 12601178
    47. Expression of human endothelial lipase in mice results in dose-dependent increase in postheparin plasma phospholipase activity, catabolic rate of HDL-apolipoprotein, and uptake of apoA-I in both kidney and liver PMID: 14517167
    48. Hepatic expression of human EL in mice resulted in markedly decreased levels of VLDL/LDL cholesterol, phospholipid, and apoB accompanied by significantly increased LDL apolipoprotein and phospholipid catabolism. PMID: 15117821
    49. N-linked glycosylation has a role in the secretion and activity of endothelial lipase PMID: 15342690
    50. results suggest that endothelial lipase (EL) on the endothelial cell surface can promote monocyte adhesion to the vascular endothelium through the interaction with heparan sulfate proteoglycans PMID: 15485805

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not
  • 数据库链接：

    HGNC: 6623

    OMIM: 603684

    KEGG: hsa:9388

    STRING: 9606.ENSP00000261292

    UniGene: Hs.465102