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  4. Human Melanoma-associated antigen D2(MAGED2) ELISA kit

Human Melanoma-associated antigen D2(MAGED2) ELISA kit

  • 中文名称：
    人黑色素瘤相关抗原D2(MAGED2)酶联免疫试剂盒
  • 货号：
    CSB-EL013352HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MAGED2 ELISA Kit was designed for the quantitative measurement of Human MAGED2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    11B6 ELISA Kit; BCG 1 ELISA Kit; BCG-1 ELISA Kit; Breast cancer-associated gene 1 protein ELISA Kit; HCA10 ELISA Kit; Hepatocellular carcinoma-associated protein JCL-1 ELISA Kit; JCL 1 ELISA Kit; MAGD2_HUMAN ELISA Kit; MAGE D ELISA Kit; MAGE D2 antigen ELISA Kit; MAGE-D2 antigen ELISA Kit; MAGED2 ELISA Kit; Melanoma antigen family D 2 ELISA Kit; Melanoma associated antigen D2 ELISA Kit; Melanoma-associated antigen D2 ELISA Kit
  • 缩写：
    MAGED2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 83-94
    1:2 Average % 97
    Range % 92-101
    1:4 Average % 93
    Range % 88-97
    1:8 Average % 107
    Range % 102-111
  • 回收率：
    The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 102-108
    EDTA plasma (n=4) 96 91-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.238 2.334 2.286 2.090
    1000 1.415 1.527 1.471 1.275
    500 0.881 0.932 0.907 0.711
    250 0.560 0.573 0.567 0.371
    125 0.379 0.381 0.380 0.184
    62.5 0.292 0.284 0.288 0.092
    31.25 0.247 0.252 0.250 0.054
    0 0.195 0.196 0.196 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
  • 基因功能参考文献：
    1. Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer. PMID: 25743330
    2. MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. PMID: 26705694
    3. We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome. PMID: 27120771
    4. these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis PMID: 22791814
    5. Expression pattern and further characterization of human MAGED2 and identification of rodent orthologues PMID: 11856887
    6. Maged2 is mainly expressed in tissues of mesodermal origin PMID: 15162511
    7. Results identified a cDNA clone, corresponding to MAGE D2 mRNA, from primary human bronchial epithelial cells which exhibits increased expression in vitro after treatment with all-trans retinoic acid. PMID: 15465002
    8. MAGED2, a novel protein, is a p53-dissociator. PMID: 17912449

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  • 相关疾病：
    Bartter syndrome 5, antenatal, transient (BARTS5)
  • 组织特异性：
    Widely expressed. In the developing and adult kidney, expressed in the thick ascending limb of the loop of Henle and the distal convoluted tubules outside the loop.
  • 数据库链接：

    HGNC: 16353

    OMIM: 300470

    KEGG: hsa:10916

    STRING: 9606.ENSP00000218439

    UniGene: Hs.522665