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MAGED2 Antibody

  • 货号：
    CSB-PA891976LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate
      All lanes: MAGED2 antibody at 4.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 65, 64 kDa
      Observed band size: 65, 64 kDa
    • IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA891976LA01HU at 1:145, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating MAGED2 in HepG2 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA891976LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA891976LA01HU (6µg) + HepG2 whole cell lysate (500µg)
      Lane 3: HepG2 whole cell lysate (20µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MAGED2 Polyclonal antibody
  • Uniprot No.：
    Q9UNF1
  • 基因名：
    MAGED2
  • 别名：
    11B6 antibody; BCG 1 antibody; BCG-1 antibody; Breast cancer-associated gene 1 protein antibody; HCA10 antibody; Hepatocellular carcinoma-associated protein JCL-1 antibody; JCL 1 antibody; MAGD2_HUMAN antibody; MAGE D antibody; MAGE D2 antigen antibody; MAGE-D2 antigen antibody; MAGED2 antibody; Melanoma antigen family D 2 antibody; Melanoma associated antigen D2 antibody; Melanoma-associated antigen D2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Melanoma-associated antigen D2 protein (38-285AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MAGED2 Antibody (CSB-PA891976LA01HU)，的标记方式是Non-conjugated。对于MAGED2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA891976LB01HU MAGED2 Antibody, HRP conjugated ELISA
    FITC CSB-PA891976LC01HU MAGED2 Antibody, FITC conjugated
    Biotin CSB-PA891976LD01HU MAGED2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
  • 基因功能参考文献：
    1. Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer. PMID: 25743330
    2. MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. PMID: 26705694
    3. We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome. PMID: 27120771
    4. these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis PMID: 22791814
    5. Expression pattern and further characterization of human MAGED2 and identification of rodent orthologues PMID: 11856887
    6. Maged2 is mainly expressed in tissues of mesodermal origin PMID: 15162511
    7. Results identified a cDNA clone, corresponding to MAGE D2 mRNA, from primary human bronchial epithelial cells which exhibits increased expression in vitro after treatment with all-trans retinoic acid. PMID: 15465002
    8. MAGED2, a novel protein, is a p53-dissociator. PMID: 17912449

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  • 相关疾病：
    Bartter syndrome 5, antenatal, transient (BARTS5)
  • 组织特异性：
    Widely expressed. In the developing and adult kidney, expressed in the thick ascending limb of the loop of Henle and the distal convoluted tubules outside the loop.
  • 数据库链接：

    HGNC: 16353

    OMIM: 300470

    KEGG: hsa:10916

    STRING: 9606.ENSP00000218439

    UniGene: Hs.522665