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  4. Human Protein notum homolog(NOTUM) ELISA kit

Human Protein notum homolog(NOTUM) ELISA kit

  • 中文名称：
    人notum蛋白同源物(NOTUM)酶联免疫试剂盒
  • 货号：
    CSB-EL015955HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human NOTUM ELISA Kit was designed for the quantitative measurement of Human NOTUM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    NOTUM ELISA Kit; notum pectinacetylesterase homolog (Drosophila) ELISA Kit; Notum pectinacetylesterase homolog ELISA Kit; NOTUM_HUMAN ELISA Kit; Protein notum homolog ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %90-100
    1:2Average %89
    Range %85-93
    1:4Average %92
    Range %88-96
    1:8Average %85
    Range %80-90
  • 回收率：
    The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-100
    EDTA plasma (n=4)106100-114
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    1002.607 2.497 2.552 2.378
    502.203 2.075 2.139 1.965
    251.635 1.542 1.589 1.415
    12.50.952 1.006 0.979 0.805
    6.250.610 0.587 0.599 0.425
    3.120.451 0.462 0.457 0.283
    1.560.332 0.315 0.324 0.150
    00.176 0.171 0.174
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
  • 基因功能参考文献：
    1. NOTUM expression was increased in metastatic cells. Proliferation was suppressed by inhibiting expression of NOTUM. Knockdown of NOTUM genes inhibited proliferation as well as migration, with possible involvement of p38 and c-JUN N-terminal kinase in this process. PMID: 30343282
    2. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
    3. Kinetic and mass spectrometric analyses of human proteins show that Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins and thus constitutes the first known extracellular protein deacylase PMID: 25731175
    4. Overexpression of NOTUM is associated with hepatocellular carcinoma PMID: 18429952
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Pectinacetylesterase family, Notum subfamily
  • 组织特异性：
    Rarely expressed in adult normal tissues.
  • 数据库链接：

    HGNC: 27106

    OMIM: 609847

    KEGG: hsa:147111

    STRING: 9606.ENSP00000387310

    UniGene: Hs.106137