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  4. Human Slit homolog 3 protein(SLIT3) ELISA kit

Human Slit homolog 3 protein(SLIT3) ELISA kit

  • 中文名称：
    人Slit同源蛋白3(SLIT3)酶联免疫试剂盒
  • 货号：
    CSB-EL021769HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human SLIT3 ELISA Kit was designed for the quantitative measurement of Human SLIT3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    FLJ10764 ELISA Kit; KIAA0814 ELISA Kit; MEGF 5 ELISA Kit; MEGF5 ELISA Kit; Multiple EGF-like domains protein 5 ELISA Kit; Multiple epidermal growth factor like domains 5 ELISA Kit; Multiple epidermal growth factor-like domains protein 5 ELISA Kit; SLIL 2 ELISA Kit; SLIL2 ELISA Kit; SLIT 1 ELISA Kit; Slit 2 ELISA Kit; Slit 3 ELISA Kit; Slit homolog 3 (Drosophila) ELISA Kit; Slit homolog 3 ELISA Kit; Slit homolog 3 protein ELISA Kit; Slit-3 ELISA Kit; SLIT1 ELISA Kit; Slit2 ELISA Kit; Slit3 ELISA Kit; SLIT3_HUMAN ELISA Kit
  • 缩写：
    SLIT3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SLIT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 90-97    
    1:2 Average % 85    
    Range % 82-93    
    1:4 Average % 110    
    Range % 105-115    
    1:8 Average % 106    
    Range % 102-117    
  • 回收率：
    The recovery of human SLIT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 86-95    
    EDTA plasma (n=4) 99 95-102    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 1.866 1.955 1.911 1.777    
    5 1.472 1.588 1.530 1.396    
    2.5 1.162 1.201 1.182 1.048    
    1.25 0.864 0.873 0.869 0.735    
    0.625 0.525 0.558 0.542 0.408    
    0.312 0.312 0.313 0.313 0.179    
    0.156 0.267 0.276 0.272 0.138    
    0 0.137 0.131 0.134 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
  • 基因功能参考文献：
    1. Overexpression of Slit3 induces its tumor suppressive effects in Breast cancer. PMID: 26542734
    2. Results show that silencing of Slit3 promoted proliferation, migration and invasion of A549 cells and induced epithelial-mesenchymal transition suggesting that Slit3 functions as a tumor suppressor in lung carcinoma. PMID: 26045181
    3. Estrogen-dependent expression of SLIT3 may play a key role in regulating nerve-vessel interactions within the complex microenvironment of endometriosis lesions. PMID: 25051436
    4. SLIT3 is increased with labour, and both amnion and myometrial studies describe a pro-inflammatory effect of SLIT3 in these tissues. PMID: 24286238
    5. High SLIT3 expression is associated with high-grade gliomas. PMID: 24158112
    6. SLIT3-ROBO4 activation promotes vascular network formation in human engineered tissue and angiogenesis in vivo. PMID: 24090675
    7. results implicate the involvement of miR-218-2 and its host gene SLIT3 in thyroid cancer cell invasion, migration, and proliferation PMID: 23720784
    8. Slit3 treatment increased the in vivo homing efficiency of CD34 HSPCs to the BM in NOD/SCID mice, whereas Slit3-exposed HSPC migration in vitro was inhibited. Results support a role for Slit3 in human HSPC migration in vitro and homing in vivo. PMID: 22614124
    9. SLIT3 duplication on 5q35.1 predisposes to major depressive disorder PMID: 21152026
    10. The chemorepellent Slit3 promotes monocyte migration. PMID: 21078908
    11. Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritis. PMID: 20298552
    12. Many variants in SLIT3 tend to associate with schizophrenia susceptibility in the Chinese Han population. PMID: 15231749
    13. data suggest that Slit proteins play a key role in guiding dorsally projecting cranial motoneurons and in facilitating their neural tube exit PMID: 16162649
    14. In fetal and embryonic stem cell cultures Slit-3 inhibited neurite outgrowth. PMID: 16840550
    15. functions to promote angiogenesis in coordinating organogenesis during embryonic development PMID: 19741192

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Predominantly expressed in thyroid.
  • 数据库链接：

    HGNC: 11087

    OMIM: 603745

    KEGG: hsa:6586

    STRING: 9606.ENSP00000430333

    UniGene: Hs.552087