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  4. Human collectin sub-family member 11,COLEC11 ELISA Kit

Human collectin sub-family member 11,COLEC11 ELISA Kit

  • 中文名称：
    人集蛋白亚家族成员11(COLEC11)酶联免疫试剂盒
  • 货号：
    CSB-E11839h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human COLEC11 ELISA Kit was designed for the quantitative measurement of Human COLEC11 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.2 pg/mL-2000 pg/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    CL K1 ELISA Kit; CL K1 I ELISA Kit; CL K1 II ELISA Kit; CL K1 IIa ELISA Kit; CL K1 IIb ELISA Kit; CL-K1 ELISA Kit; CLK1 ELISA Kit; COL11_HUMAN ELISA Kit; COLEC 11 ELISA Kit; COLEC11 ELISA Kit; Collectin 11 ELISA Kit; Collectin kidney I ELISA Kit; Collectin kidney protein 1 ELISA Kit; Collectin sub family member 11 ELISA Kit; Collectin-11 ELISA Kit; Collectin11 ELISA Kit; DKFZp686N1868 ELISA Kit; MGC129470 ELISA Kit; MGC129471 ELISA Kit; MGC3279 ELISA Kit
  • 缩写：
    COLEC11
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.156 ng/ml-10 ng/ml
  • 灵敏度：
    0.039 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 80-92  
    1:2 Average % 99  
    Range % 91-110  
    1:4 Average % 97  
    Range % 92-105  
    1:8 Average % 93  
    Range % 90-98  
  • 回收率：
    The recovery of human COLEC11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-100  
    EDTA plasma (n=4) 90 86-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.649 2.462 2.556 2.433  
    5 2.167 2.054 2.111 1.988  
    2.5 1.477 1.385 1.431 1.308  
    1.25 0.821 0.789 0.805 0.682  
    0.625 0.450 0.432 0.441 0.318  
    0.312 0.278 0.269 0.274 0.151  
    0.156 0.207 0.202 0.205 0.082  
    0 0.124 0.121 0.123 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Lectin that plays a role in innate immunity, apoptosis and embryogenesis. Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope. Primarily recognizes the terminal disaccharide of the glycan. Also recognizes a subset of fucosylated glycans and lipopolysaccharides. Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1. Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding. Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis.
  • 基因功能参考文献：
    1. this study demonstrates the role of CL-11 and the molecular mechanisms involved in modulating retinal pigment epithelial cell phagocytosis and cytokine production PMID: 28772263
    2. High CLK1 expression is associated with cardiovascular disease. PMID: 27341702
    3. The exclusion of the MASP1 and COLEC11 Loci in two individuals from different consanguineous families and the absence of mutations in four further individuals sequenced for both genes raises the possibility that that there is further genetic heterogeneity of 3MC syndrome PMID: 26789649
    4. identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacterial mannose-capped lipoarabinomannan as a primary ligand for CL-LK PMID: 26173080
    5. Data indicate differences in the plasma concentrations of collectin liver 1 and collectin kidney 1, M-ficolin and H-ficol in systemic lupus erythematosus (SLE) patients compared to a group of healthy controls. PMID: 26154564
    6. promoter polymorphism COLEC11-9570C>T (rs3820897) was associated with decreased levels of CL-K1. PMID: 25710878
    7. the sugar specificity of CL-K1 was established. PMID: 25912189
    8. These results suggest that specific diseases may affect CL-K1 synthesis in an organ dependent manner and that elevated plasma CL-K1 levels are associated with the presence of DIC. PMID: 24474086
    9. Most plasma CL-K1 was found in complex with CL-L1 in a ratio suggesting a heteromeric subunit of 1 CL-L1 & 2 CL-K1 polypeptides. It associated with MASPs 1, 2 & 3. On binding mannan or DNA in the presence of MASP-2, the complex deposited C4b. PMID: 24174618
    10. Data suggest that collectin 11 (CL-11), e.g. via complement, may play a role in response to particles and surfaces presenting extracellular DNA, such as apopototic cells. PMID: 23954398
    11. collectin-11 associates with all the known MBL-associated serine proteases (MASP-1, MASP-2 and MASP-3) as well as the lectin complement pathway regulator MAP-1. PMID: 23220946
    12. Data show that the concentration of collectin kidney 1 (CL-K1, COLEC11) in plasma was 0.34 +/- 0.13 microg/ml and that in mannan-binding lectin (MBL) was 1.72 +/- 1.51 microg/ml. PMID: 21893516
    13. these findings demonstrate a role for complement pathway factors in fundamental developmental processes and in the etiology of 3MC syndrome. PMID: 21258343
    14. CL-11 plays a role in activation of the complement system and in the defense against invading microorganisms PMID: 20956340

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  • 相关疾病：
    3MC syndrome 2 (3MC2)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    COLEC10/COLEC11 family
  • 组织特异性：
    Ubiquitous. Detected in adrenal gland, kidney, liver, ovaries and testis (at protein level).
  • 数据库链接：

    HGNC: 17213

    OMIM: 265050

    KEGG: hsa:78989

    STRING: 9606.ENSP00000339168

    UniGene: Hs.32603