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Recombinant Human Collectin-11 (COLEC11)

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  • 中文名称：
    Recombinant Human Collectin-11(COLEC11)
  • 货号：
    CSB-MP861154HU
  • 规格：
    ￥3960
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP861154HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) COLEC11.
    • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP861154HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) COLEC11.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    COLEC11
  • Uniprot No.：
  • 别名：
    CL K1; CL K1 I; CL K1 II; CL K1 IIa; CL K1 IIb; CL-K1; CLK1; COL11_HUMAN; COLEC 11; COLEC11; Collectin 11; Collectin kidney I; Collectin kidney protein 1; Collectin sub family member 11; Collectin-11; Collectin11; DKFZp686N1868; MGC129470; MGC129471; MGC3279
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Mammalian cell
  • 分子量：
    30.1kDa
  • 表达区域：
    26-271aa
  • 氨基酸序列
    QPAGDDACSVQILVPGLKGDAGEKGDKGAPGRPGRVGPTGEKGDMGDKGQKGSVGRHGKIGPIGSKGEKGDSGDIGPPGPNGEPGLPCECSQLRKAIGEMDNQVSQLTSELKFIKNAVAGVRETESKIYLLVKEEKRYADAQLSCQGRGGTLSMPKDEAANGLMAAYLAQAGLARVFIGINDLEKEGAFVYSDHSPMRTFNKWRSGEPNNAYDEEDCVEMVASGGWNDVACHTTMYFMCEFDKENM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    To make this Recombinant Human COLEC11 protein, the COLEC11 gene was isolated at first and cloned into an expression vector. CUSABIO has built a mature recombinant protein platform. This Recombinant Human COLEC11 protein was developed in the platform. It was expressed in Mammalian cell at the region of 26-271aa of the Human COLEC11 protein. N-terminal 10xHis tag and C-terminal Myc tag was fused with the expression vector for affinity and purification purposes. The purity is 90%+ determined by SDS-PAGE.

    The gene encoding collectin-11, COLEC11, is located on chromosome 2p25.3 (OMIM 612502) and comprises 7 exons that transcribe the canonical protein. COLEC11 variability was shown to interfere with expression and also with the binding of calcium and carbohydrates, possibly affecting protein folding. Collectin-11 shows a strong binding affinity to fucose-proteins, as found in the Tc-85 protein family, expressed on the surface of T. cruzi metacyclic trypomastigotes. Those proteins are involved in the entry of the parasite to host cells. In addition, collectin-11 is structurally similar to MBL and it has been shown that both MBL levels and MBL2 genetic variants were associated with disease susceptibility and pathophysiology of CD. Considering these observations, collectin-11 plasma levels and COLEC11 variants in exon 7 were assessed to investigate their potential role in the chronic CD. Moreover, on account of the interaction between collectin-11 and MASPs for complement activation, gene-gene interaction between COLEC11 and MASP2 was assessed to evaluate the additive genetic effect of the two loci and their role in the pathophysiology of this chronic disease.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lectin that plays a role in innate immunity, apoptosis and embryogenesis. Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope. Primarily recognizes the terminal disaccharide of the glycan. Also recognizes a subset of fucosylated glycans and lipopolysaccharides. Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1. Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding. Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis.
  • 基因功能参考文献：
    1. this study demonstrates the role of CL-11 and the molecular mechanisms involved in modulating retinal pigment epithelial cell phagocytosis and cytokine production PMID: 28772263
    2. High CLK1 expression is associated with cardiovascular disease. PMID: 27341702
    3. The exclusion of the MASP1 and COLEC11 Loci in two individuals from different consanguineous families and the absence of mutations in four further individuals sequenced for both genes raises the possibility that that there is further genetic heterogeneity of 3MC syndrome PMID: 26789649
    4. identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacterial mannose-capped lipoarabinomannan as a primary ligand for CL-LK PMID: 26173080
    5. Data indicate differences in the plasma concentrations of collectin liver 1 and collectin kidney 1, M-ficolin and H-ficol in systemic lupus erythematosus (SLE) patients compared to a group of healthy controls. PMID: 26154564
    6. promoter polymorphism COLEC11-9570C>T (rs3820897) was associated with decreased levels of CL-K1. PMID: 25710878
    7. the sugar specificity of CL-K1 was established. PMID: 25912189
    8. These results suggest that specific diseases may affect CL-K1 synthesis in an organ dependent manner and that elevated plasma CL-K1 levels are associated with the presence of DIC. PMID: 24474086
    9. Most plasma CL-K1 was found in complex with CL-L1 in a ratio suggesting a heteromeric subunit of 1 CL-L1 & 2 CL-K1 polypeptides. It associated with MASPs 1, 2 & 3. On binding mannan or DNA in the presence of MASP-2, the complex deposited C4b. PMID: 24174618
    10. Data suggest that collectin 11 (CL-11), e.g. via complement, may play a role in response to particles and surfaces presenting extracellular DNA, such as apopototic cells. PMID: 23954398
    11. collectin-11 associates with all the known MBL-associated serine proteases (MASP-1, MASP-2 and MASP-3) as well as the lectin complement pathway regulator MAP-1. PMID: 23220946
    12. Data show that the concentration of collectin kidney 1 (CL-K1, COLEC11) in plasma was 0.34 +/- 0.13 microg/ml and that in mannan-binding lectin (MBL) was 1.72 +/- 1.51 microg/ml. PMID: 21893516
    13. these findings demonstrate a role for complement pathway factors in fundamental developmental processes and in the etiology of 3MC syndrome. PMID: 21258343
    14. CL-11 plays a role in activation of the complement system and in the defense against invading microorganisms PMID: 20956340

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  • 相关疾病：
    3MC syndrome 2 (3MC2)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    COLEC10/COLEC11 family
  • 组织特异性：
    Ubiquitous. Detected in adrenal gland, kidney, liver, ovaries and testis (at protein level).
  • 数据库链接：

    HGNC: 17213

    OMIM: 265050

    KEGG: hsa:78989

    STRING: 9606.ENSP00000339168

    UniGene: Hs.32603