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  4. Human hepatocyte growth factor activator(HGFA) ELISA kit

Human hepatocyte growth factor activator(HGFA) ELISA kit

  • 中文名称：
    人肝细胞生长因子激活物(HGFA)酶联免疫试剂盒
  • 货号：
    CSB-E13160h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human HGFAC ELISA Kit was designed for the quantitative measurement of Human HGFAC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 别名：
    HGFAC ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
  • 缩写：
    HGFAC
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %81-89
    1:2Average %95
    Range %91-99
    1:4Average %87
    Range %82-93
    1:8Average %105
    Range %101-109
  • 回收率：
    The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-91
    EDTA plasma (n=4)9491-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.117 2.148 2.133 2.025
    25001.696 1.682 1.689 1.581
    12501.215 1.268 1.242 1.134
    6250.834 0.816 0.825 0.717
    3120.502 0.536 0.519 0.411
    1560.265 0.258 0.262 0.154
    780.168 0.171 0.170 0.062
    00.106 0.109 0.108
  • 数据处理：
  • 货期：
    3-5 working days

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靶点详情

  • 功能：
    Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
  • 基因功能参考文献：
    1. the HGF-activator has a role in multiple myeloma cells' catalysis of hepatocyte growth factor (HGF) activation PMID: 15172968
    2. HGF/MET signaling and aberrant HGF-activator expression is associated with diffuse large B-cell lymphoma PMID: 16189274
    3. KLK4 and KLK5 activate pro-HGFA. PMID: 18221492
    4. Results suggest that hepatocyte growth factor activator is a major serum activator of pro-macrophage-stimulating protein. PMID: 19456860
    5. HAI-1 (serine peptidase inhibitor, Kunitz type 1) specifically traps active form of HGF activator on cellular surface and regulates HGF activation in pericellular microenvironment. PMID: 11013244

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  • 亚细胞定位：
    Secreted. Note=Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Liver.
  • 数据库链接：

    HGNC: 4894

    OMIM: 604552

    KEGG: hsa:3083

    STRING: 9606.ENSP00000372224

    UniGene: Hs.104