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  4. Human protein disulfide-isomerase A3,PDIA3 ELISA Kit

Human protein disulfide-isomerase A3,PDIA3 ELISA Kit

  • 中文名称：
    人蛋白二硫化物异构酶A3(PDIA3)酶联免疫试剂盒
  • 货号：
    CSB-E09798h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PDIA3 ELISA Kit was designed for the quantitative measurement of Human PDIA3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

  • 别名：
    58 kDa glucose regulated protein ELISA Kit; 58 kDa glucose-regulated protein ELISA Kit; 58 kDa microsomal protein ELISA Kit; Disulfide isomerase ER 60 ELISA Kit; Disulfide isomerase ER-60 ELISA Kit; Endoplasmic reticulum resident protein 57 ELISA Kit; Endoplasmic reticulum resident protein 60 ELISA Kit; ER p57 ELISA Kit; ER protein 57 ELISA Kit; ER protein 60 ELISA Kit; ERp 57 ELISA Kit; ERp57 ELISA Kit; ERp60 ELISA Kit; ERp61 ELISA Kit; Glucose Regulated Protein 58 Kd ELISA Kit; GRP 57 ELISA Kit; GRP 58 ELISA Kit; GRP57 ELISA Kit; HsT17083 ELISA Kit; p58 ELISA Kit; PDIA 3 ELISA Kit; PDIA3 ELISA Kit; PDIA3_HUMAN ELISA Kit; Phospholipase C alpha ELISA Kit; PI PLC ELISA Kit; Protein disulfide isomerase A3 ELISA Kit; Protein disulfide isomerase family A member 3 ELISA Kit; Protein disulfide-isomerase A3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    1.25 ng/mL-80 ng/mL
  • 灵敏度：
    0.31 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %87-98
    1:2Average %104
    Range %98-108
    1:4Average %92
    Range %85-96
    1:8Average %97
    Range %91-102
  • 回收率：
    The recovery of hhuman PDIA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9590-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.576 2.521 2.549 2.398
    402.156 2.054 2.105 1.954
    201.487 1.445 1.466 1.315
    100.766 0.777 0.772 0.621
    50.492 0.468 0.480 0.329
    2.50.374 0.369 0.372 0.221
    1.250.255 0.248 0.252 0.101
    00.153 0.149 0.151
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. Data suggest that disulfide bond switch in allosteric activation/inactivation of TGM2 is example of post-translational redox regulation that is reversibly and allosterically modulated by two proteins (ERp57 and TRX). (TGM2 = transglutaminase 2; ERp57 = endoplasmic reticulum resident protein 57; TRX = thioredoxin-1) PMID: 29305423
    2. Study demonstrated that the expression of PDIA3 is frequently upregulated at the mRNA and protein levels in diffuse gliomas. Its high expression was significantly correlated with high Ki-67, more TP53 mutations and poor survival outcome. These findings imply that high expression of PDIA3 plays an important role in diffuse glioma progression. PMID: 29207176
    3. summarize available knowledge of ERp57's functions in subcellular compartments and the roles of dysregulated ERp57 in various diseases. PMID: 28723413
    4. ERp57 is involved in sperm capacitation and spermatozoa-zona pellucida binding. PMID: 28175305
    5. Thiol isomerase ERp57 is mobilized to the surface of activated platelets via a process that requires actin polymerization. PMID: 27079884
    6. Findings suggested that miR-330-5p represents a potential tumor-suppressive miRNA and plays an important role in cutaneous malignant melanoma progression by suppressing TYR and PDIA3 expression. PMID: 27363653
    7. Our study found increased expression of ERp57 in chronic hepatitis B -hepatocellular carcinoma (HBV-HCC) genesis. Such altered expression could be related to HBV infection and high ERp57 expression may lead to poor prognosis of HBV-HCC patients. PMID: 28373975
    8. The interaction of different flavonoids with PDIA3 was investigated by quenching fluorescence analysis and the effects on protein activity were evaluated. PMID: 28044092
    9. Upregulation of PDIA3 promotes ovarian cancer cell proliferation and migration. PMID: 27492604
    10. Data show that knockdown of endoplasmic reticulum resident protein 57 (ERP57) expression resulted in increase in shikonin induced apoptosis, and suggest that ERP57 might be a therapeutic option for the treatment of acute myeloid leukemia. PMID: 27415599
    11. Amyotrophic lateral sclerosis-linked PDIA3 mutations disrupt motor neuron connectivity. PMID: 26869642
    12. These data indicate that the presence of autoantibodies to PDIA3 favors the development of an efficient and specific T-cell response against PDIA3 in colorectal cancer patients. PMID: 26772958
    13. ERp57 levels are increased in the airway epithelium of asthmatic patients and in mice with allergic airways disease. PMID: 26435004
    14. LEDGF/p75 Overexpression Attenuates Oxidative Stress-Induced Necrosis and Upregulates the Oxidoreductase ERP57/PDIA3/GRP58 in Prostate Cancer PMID: 26771192
    15. it is possible that the vimentin and PDIA3 could be the candidate biomarkers specific to Achyranthes saponin therapy for rheumatoid arthritis in synovial membrane. PMID: 26724776
    16. ERp57 specifically contributes to peripheral nerve regeneration, whereas its activity is dispensable for the survival of a specific neuronal population of the central nervous system. PMID: 26361352
    17. Data suggest that regulation of PDIA3 (protein disulfide isomerase associated 3 protein) expression by microRNA-330-5p is important in maintaining hair cycle through regulation of proliferation and migration capability of keratinocytes. PMID: 26402295
    18. miR148a inhibits the proliferation and promotes paclitaxelinduced apoptosis of ovarian cancer cells, and this may be partly attributed to direct targeting of PDIA3. PMID: 26004124
    19. results suggested that ER chaperone PDIA3 plays a pivotal role in FFA-induced hepatocyte steatosis and apoptosis. PMID: 26214517
    20. The sPLA2-hPDI model constitutes a valuable tool to facilitate further insights into this process and into the (patho)physiology of sPLA2s in relation to their action intracellularly. PMID: 25763817
    21. Its cross-reaction to GFAP antibodies seems to lead to a changed signaling in the cells triggering the protective effects. PMID: 25837926
    22. PDIA3 and PDIA6 gene expression is a neoplasm aggressiveness marker in primary ductal breast cancer. PMID: 26125904
    23. study identifies ERp57 as a new modulator of PrP levels and may help with understanding the consequences of ERp57 up-regulation observed in human disease PMID: 26170458
    24. Reported increased expression of ALDH3A1, PDIA3, and PRDX2 in pterygia using a proteomic approach. These proteins are presumed to have a protective role against oxidative stress-induced apoptosis. PMID: 25221425
    25. Suggest that ERp57-STAT3 regulation functions in radioresistance of laryngeal cancer. PMID: 25605256
    26. Increased expression of PDIA3 is associated with tumor progression and the clinical behavior of gallbladder carcinoma. PMID: 23782473
    27. Results demonstrated that Grp58 appears to regulate WNT signaling by targeting beta-catenin to augment cancer invasion. PMID: 25081282
    28. Increased expression of the AGR2 and ERp57 is associated with cancer. [review] PMID: 24490732
    29. ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake PMID: 24815697
    30. Low ERp57 expression independently predicts a poor outcome for patients with cervical cancer. PMID: 23957851
    31. ERP57 was excreted in the early stages of chronic kidney disease, and its level in urine correlated with the degree of renal fibrosis, suggesting that the secretion of ERP57 represents one of the first signs of renal fibrosis onset and progression. PMID: 23781031
    32. Tg "adduct B" was identified as primarily engaging the endoplasmic reticulum oxidoreductases ERp57 and protein disulfide isomerase PMID: 24599957
    33. Low PDIA3 expression is associated with chemoresistant colon tumors. PMID: 23255428
    34. ERp57 appears as a multifunctional chaperone that can regulate diverse biological processes to maintain the homeostasis of breast cancer cells and promote the development of bone metastasis. PMID: 23625662
    35. ERp57 positively regulates H(+),K(+)-ATPase activity apart from its chaperoning function. PMID: 24188822
    36. Platelets lacking ERp57 have defective activation of the alphaIIbbeta3 integrin and platelet aggregation. The defect in aggregation was corrected by the addition of exogenous ERp57, implicating surface ERp57 in platelet aggregation. PMID: 24030382
    37. We postulate that apoB cleavage by ER-60 within the ER lumen could facilitate proteasomal degradation of the C-terminus of translocationally-arrested apoB. PMID: 23827315
    38. Nuclear ERp57 interacts in vivo with DNA fragments in melanoma cells and is potentially involved in the transcriptional regulation of its target genes. PMID: 23587917
    39. ERp57 regulates RalA signalling by acting as a redox-sensitive guanine-nucleotide dissociation inhibitor. PMID: 23226417
    40. Data indicate that the peptide-loading complex (PLC) consists maximally of 2x tapasin-ERp57/MHC I per TAP complex, but one tapasin-ERp57/MHC I in the PLC is essential and sufficient for antigen processing. PMID: 22923333
    41. Downregulation of ER60 protease is associated with inhibition of cell proliferation in breast cancer cells in vitro. PMID: 22266712
    42. PDIA3 may play in the progression of heart failure. PMID: 22503978
    43. the vitamin D receptor (VDR) and ERp57 are critical components for actions of 1,25 dihydroxy vitamin D 3 against DNA damage, but the VDR does not require normal DNA binding or classical ligand binding to mediate photoprotection. PMID: 22322599
    44. kinetic, extent and stability of the ERp57-CRT complex, using the surface plasmon resonance spectroscopy PMID: 21996511
    45. ERp57 has roles in multiple components of platelet function and arterial thrombus formation. PMID: 22168334
    46. These results suggest that increased level of hnRNP-H and PDIA3 expression in Dengue virus infected THP1 cells assist in the viral replication by suppressing the TNF-alpha production. PMID: 22207023
    47. PDIA3 and GRP78 are IL11 targets in invasive trophoblasts PMID: 21840908
    48. Grp58 is highly expressed in patients with serious cervical stromal penetration, and further supports the theory that Grp58 is critical for cervical AD progression. PMID: 21917082
    49. ERp57 participates in signal transduction from the cell surface, in regulatory processes taking place in the nucleus, and in multimeric protein complexes involved in DNA repair. (Review) PMID: 21837552
    50. Immunostaining for both ERp57-GFP and p65 after TNF-alpha treatment indicated that nuclear translocation of these two proteins occurs independently PMID: 21598303

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  • 亚细胞定位：
    Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 组织特异性：
    Detected in the flagellum and head region of spermatozoa (at protein level). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level).
  • 数据库链接：

    HGNC: 4606

    OMIM: 602046

    KEGG: hsa:2923

    STRING: 9606.ENSP00000300289

    UniGene: Hs.591095