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Mouse R-spondin-3(RSPO3) ELISA kit

  • 中文名称：
    小鼠R脊椎蛋白3(RSPO3)酶联免疫试剂盒
  • 货号：
    CSB-EL020552MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse RSPO3 ELISA Kit was designed for the quantitative measurement of Mouse RSPO3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

  • 别名：
    Rspo3 ELISA Kit; R-spondin-3 ELISA Kit; Cabriolet ELISA Kit; Cysteine-rich and single thrombospondin domain-containing protein 1 ELISA Kit; Cristin-1 ELISA Kit; Nucleopondin ELISA Kit; Roof plate-specific spondin-3 ELISA Kit
  • 缩写：
    RSPO3
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.17 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-94
    1:2Average %98
    Range %93-103
    1:4Average %102
    Range %98-105
    1:8Average %94
    Range %92-96
  • 回收率：
    The recovery of mouse RSPO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9794-100
    EDTA plasma (n=4)9390-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    3002.693 2.800 2.747 2.597
    1501.811 1.970 1.891 1.741
    751.073 1.135 1.104 0.954
    37.50.652 0.701 0.677 0.527
    18.750.369 0.375 0.372 0.222
    9.40.247 0.266 0.257 0.107
    4.70.202 0.214 0.208 0.058
    00.158 0.142 0.150
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.
  • 基因功能参考文献：
    1. These results shed new light on the role of Rspo3 in heart development and demonstrate that LGR4 is the principal R-spondin 3 receptor in the heart. PMID: 29859889
    2. Results identify a mechanism through which localized expression of RSPO3 induces proliferation of the coronary arteries at their stems and permits their formation. PMID: 28834739
    3. Rspo3-LGR4 axis protects hepatocytes from hypoxia/reoxygenation injury via activating beta-catenin. PMID: 29555474
    4. H. pylori infection increases stromal R-spondin 3 expression and expands the Axin2(+) cell pool to cause hyperproliferation and gland hyperplasia; the ability of stromal niche cells to control and adapt epithelial stem cell dynamics constitutes a sophisticated mechanism that orchestrates epithelial regeneration and maintenance of tissue integrity PMID: 28813421
    5. We provide in vivo evidence that RSPO3 stimulates the crypt stem cell and niche compartments and drives rapid intestinal tumorigenesis. PMID: 27511199
    6. Rspo3 function remains essential in adult life to ensure replenishment of lost cells and maintain the properties of the zona glomerulosa PMID: 27313319
    7. Conditional deletion of Rspo3 in mice disrupts activation of central fate, demonstrating its crucial role in determining and maintaining beta-catenin-dependent zonation. PMID: 26655896
    8. data identify endothelial RSPO3-driven non-canonical WNT/Ca(2+)/NFAT signaling as a critical maintenance pathway of the remodeling vasculature PMID: 26766444
    9. Nkx2-5 has a role in regulating cardiac growth through modulation of Wnt signaling by R-spondin3 PMID: 25053429
    10. Rspo3 loss of function mutation in combination with Rspo2 mutation,but not on its own, causes severe limb truncation. PMID: 22610508
    11. Sexually dimorphic expression patterns were observed for WNT/beta-catenin-responsive genes, and for Wnt2b, Wnt4, Wnt7a, Wnt9b, Wnt10b, Wnt11, Wnt16, and Rspo3 mRNAs. PMID: 21936019
    12. Data show that Rspo3 binds syndecan 4 and that together they activate Wnt5a/PCP signaling. PMID: 21397842
    13. suggest a critical role for Rspo3 in the interaction between chorion and allantois in labyrinthine development PMID: 16963017
    14. identify Rspo3 as a novel, evolutionarily conserved angiogenic factor in embryogenesis PMID: 18842812

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    R-spondin family
  • 组织特异性：
    Highly expressed in endothelial cells.
  • 数据库链接：