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  4. Recombinant Mouse R-spondin-3 (Rspo3)

Recombinant Mouse R-spondin-3 (Rspo3)

  • 货号：
    CSB-YP650357MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP650357MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP650357MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP650357MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP650357MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rspo3
  • Uniprot No.：
  • 别名：
    Rspo3; R-spondin-3; Cabriolet; Cysteine-rich and single thrombospondin domain-containing protein 1; Cristin-1; Nucleopondin; Roof plate-specific spondin-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-277
  • 氨基酸序列
    QNASRGRRQ RRMHPNVSQG CQGGCATCSD YNGCLSCKPR LFFVLERIGM KQIGVCLSSC PSGYYGTRYP DINKCTKCKV DCDTCFNKNF CTKCKSGFYL HLGKCLDSCP EGLEANNHTM ECVSIVHCEA SEWSPWSPCM KKGKTCGFKR GTETRVRDIL QHPSAKGNLC PPTSETRTCI VQRKKCSKGE RGKKGRERKR KKLNKEERKE TSSSSDSKGL ESSIETPDQQ ENKERQQQQK RRARDKQQKS VSVSTVH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.
  • 基因功能参考文献：
    1. These results shed new light on the role of Rspo3 in heart development and demonstrate that LGR4 is the principal R-spondin 3 receptor in the heart. PMID: 29859889
    2. Results identify a mechanism through which localized expression of RSPO3 induces proliferation of the coronary arteries at their stems and permits their formation. PMID: 28834739
    3. Rspo3-LGR4 axis protects hepatocytes from hypoxia/reoxygenation injury via activating beta-catenin. PMID: 29555474
    4. H. pylori infection increases stromal R-spondin 3 expression and expands the Axin2(+) cell pool to cause hyperproliferation and gland hyperplasia; the ability of stromal niche cells to control and adapt epithelial stem cell dynamics constitutes a sophisticated mechanism that orchestrates epithelial regeneration and maintenance of tissue integrity PMID: 28813421
    5. We provide in vivo evidence that RSPO3 stimulates the crypt stem cell and niche compartments and drives rapid intestinal tumorigenesis. PMID: 27511199
    6. Rspo3 function remains essential in adult life to ensure replenishment of lost cells and maintain the properties of the zona glomerulosa PMID: 27313319
    7. Conditional deletion of Rspo3 in mice disrupts activation of central fate, demonstrating its crucial role in determining and maintaining beta-catenin-dependent zonation. PMID: 26655896
    8. data identify endothelial RSPO3-driven non-canonical WNT/Ca(2+)/NFAT signaling as a critical maintenance pathway of the remodeling vasculature PMID: 26766444
    9. Nkx2-5 has a role in regulating cardiac growth through modulation of Wnt signaling by R-spondin3 PMID: 25053429
    10. Rspo3 loss of function mutation in combination with Rspo2 mutation,but not on its own, causes severe limb truncation. PMID: 22610508
    11. Sexually dimorphic expression patterns were observed for WNT/beta-catenin-responsive genes, and for Wnt2b, Wnt4, Wnt7a, Wnt9b, Wnt10b, Wnt11, Wnt16, and Rspo3 mRNAs. PMID: 21936019
    12. Data show that Rspo3 binds syndecan 4 and that together they activate Wnt5a/PCP signaling. PMID: 21397842
    13. suggest a critical role for Rspo3 in the interaction between chorion and allantois in labyrinthine development PMID: 16963017
    14. identify Rspo3 as a novel, evolutionarily conserved angiogenic factor in embryogenesis PMID: 18842812

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    R-spondin family
  • 组织特异性：
    Highly expressed in endothelial cells.
  • 数据库链接：

    KEGG: mmu:72780

    STRING: 10090.ENSMUSP00000090287

    UniGene: Mm.45191