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  4. Mouse coagulation factor Ⅴ,FⅤ ELISA Kit

Mouse coagulation factor Ⅴ,FⅤ ELISA Kit

  • 中文名称：
    小鼠凝血因子Ⅴ(FⅤ)酶联免疫试剂盒
  • 货号：
    CSB-E17815m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse Coagulation factor V (F5) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor V (F5) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

  • 别名：
    F5Coagulation factor V ELISA Kit; Activated protein C cofactor) [Cleaved into: Coagulation factor V heavy chain; Coagulation factor V light chain] ELISA Kit
  • 缩写：
    FⅤ
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    78 ng/mL-5000 ng/mL
  • 灵敏度：
    19.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %96
    Range %91-103
    1:200Average %100
    Range %95-104
    1:400Average %95
    Range %90-100
    1:800Average %98
    Range %94-105
  • 回收率：
    The recovery of mouse FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-96
    EDTA plasma (n=4)8884-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    50002.351 2.521 2.436 2.240
    25001.823 1.907 1.865 1.669
    12501.354 1.294 1.324 1.128
    6250.853 0.893 0.873 0.677
    3120.612 0.607 0.610 0.414
    1560.401 0.421 0.411 0.215
    780.298 0.287 0.293 0.097
    00.200 0.192 0.196
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
  • 基因功能参考文献：
    1. Mice with the FVL mutation do not have increased spermatogenesis as compared to wildtype mice. PMID: 28927238
    2. Platelet-derived FV is a critical mediator of arterial thrombosis that involves platelet activation. PMID: 28673898
    3. Platelet-derived FV contributes to the control of angiogenesis and is likely associated with thrombin generation in hind limb ischemia model. PMID: 27237401
    4. Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of alpha1-antitrypsin. PMID: 21795745
    5. The FVL mutation does not influence coagulation activation, lung inflammation or survival in lethal influenza A. PMID: 20413539
    6. It suggested that there could be a combination of GLA deficiency and FVL or other thrombosis-related gene defect in patients with genetic severe early-onset thrombosis. PMID: 20533258
    7. that the murine platelet FV compartment is derived exclusively from primary biosynthesis within cells of marrow origin and an intact platelet FV pool is not required for protection from spontaneous hemorrhage or bleeding following minor trauma PMID: 12816857
    8. the murine platelet and plasma FV pools are biosynthetically distinct PMID: 12855561
    9. The source of the FVL leading to accelerated thrombosis appears to be circulating, non-platelet-derived plasma FVL. PMID: 15809370
    10. FVL has the ability to improve the hemophilia A or B phenotype, but this effect is principally evident at the microcirculation level following a particular vascular injury. PMID: 16359510
    11. observations demonstrate a synergistic interaction between alpha-galactosidase A deficiency and Factor V Leiden toward tissue fibrin deposition; concomitant mutations in these genes may increase the penetrance of vascular thrombotic events in humans PMID: 16514103
    12. Fetal gene defects precipitate platelet-mediated pregnancy failure in factor V Leiden mothers. PMID: 17438064
    13. The FVL mutation has no effect on the development of secondary tumours of colon cancer in livers of mice. PMID: 17635646
    14. FV Leiden allele has no effect in murine colitis and we thus question the importance of activated blood coagulation in the etiology or pathogenesis of inflammatory bowel disease. PMID: 17849058
    15. the factor V Leiden mutation is associated with enhanced thrombotic tendency after FeCl3 injury of the femoral artery on a standard fat diet, but not a high fat diet PMID: 17938812
    16. In contrast to humans, pregnancy induced a decrease in APC resistance in wild-type and in FV Leiden mice. PMID: 19036061
    17. Homozygous Factor V could promote atherosclerosis and fibrin deposition in apolipoprotein E deficient mice. PMID: 19671356

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Multicopper oxidase family
  • 数据库链接：