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  4. Pig Lactoferrin(LF/LTF)ELISA kit

Pig Lactoferrin(LF/LTF)ELISA kit

  • 中文名称：
    猪乳铁传递蛋白/乳铁蛋白(LF/LTF)酶联免疫试剂盒
  • 货号：
    CSB-E16230p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig LTF ELISA Kit was designed for the quantitative measurement of Pig LTF protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.078 μg/mL.

  • 别名：
    LTF ELISA Kit; Lactotransferrin ELISA Kit; Lactoferrin ELISA Kit; EC 3.4.21.- ELISA Kit
  • 缩写：
    LTF
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.156 μg/mL-10 μg/mL
  • 灵敏度：
    0.078 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig LF/LTFin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 103  
    Range % 99-107  
    1:2 Average % 85  
    Range % 81-89  
    1:4 Average % 98  
    Range % 94-102  
    1:8 Average % 91  
    Range % 87-95  
  • 回收率：
    The recovery of pig LF/LTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 91-102  
    EDTA plasma (n=4) 86 80-92  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.135 0.137 0.136    
    5 0.224 0.215 0.220    
    2.5 0.348 0.353 0.351    
    1.25 0.576 0.568 0.572    
    0.625 1.006 1.021 1.014    
    0.312 1.587 1.671 1.629    
    0.156 1.998 1.854 1.926    
    0 2.354 2.228 2.291    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.; Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling.; The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites.
  • 基因功能参考文献：
    1. this paper shows that recombinant lactoferrin peptide LF-6 produced with intein technology has both antibacterial and immunomodulatory activity in mice PMID: 27487204
    2. We propose that lactoferrin functions in the immune defense of the small intestinal mucosa by targeting the population of T cells in the lamina propria PMID: 20607309
    3. Involvement of HSP27 and lactoferrin in porcine endometrium and neutrophils activity. PMID: 20541878
    4. Growth performance of animal pups is enhanced by directly feeding the genetically engineered milk containing enriched pLF protein in transgenic mice. PMID: 17489602
  • 亚细胞定位：
    Secreted. Cytoplasmic granule.
  • 蛋白家族：
    Transferrin family
  • 数据库链接：