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Pig soluble P-selectin,sP-Selectin ELISA Kit

  • 中文名称:
    猪可溶性P选择素(sP-Selectin)酶联免疫试剂盒
  • 货号:
    CSB-E08106p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Pig sP-Selectin ELISA Kit was designed for the quantitative measurement of Pig sP-Selectin protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

  • 缩写:
    sP-Selectin
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    12.5 ng/mL-800 ng/mL
  • 灵敏度:
    3.12 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %85-94
    1:2Average %95
    Range %92-98
    1:4Average %87
    Range %82-90
    1:8Average %93
    Range %90-96
  • 回收率:
    The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8783-90
    EDTA plasma (n=4)8582-89
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8001.828 1.968 1.898 1.750
    4001.179 1.236 1.208 1.060
    2000.734 0.757 0.746 0.598
    1000.485 0.476 0.481 0.333
    500.311 0.323 0.317 0.169
    250.251 0.257 0.254 0.106
    12.50.188 0.182 0.185 0.037
    00.145 0.151 0.148
  • 数据处理:
  • 货期:
    3-5 working days

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