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  4. Rat oncostatin M (OSM) ELISA kit

Rat oncostatin M (OSM) ELISA kit

  • 中文名称：
    大鼠抑瘤素M(OSM)酶联免疫试剂盒
  • 货号：
    CSB-E07402r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat OSM ELISA Kit was designed for the quantitative measurement of Rat OSM protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Osm ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-95
    1:2 Average % 104
    Range % 99-108
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 95
    Range % 91-99
  • 回收率：
    The recovery of rat OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 92-102
    EDTA plasma (n=4) 89 82-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.877 2.986 2.932 2.828
    500 2.315 2.421 2.368 2.264
    250 1.531 1.567 1.549 1.445
    125 0.818 0.824 0.821 0.717
    62.5 0.403 0.412 0.408 0.304
    31.25 0.272 0.265 0.269 0.165
    15.6 0.206 0.202 0.204 0.100
    0 0.103 0.104 0.104 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
  • 基因功能参考文献：
    1. OSM is a key mediator for inducing differentiation of OC15-5 cells into hepatocytes PMID: 15743783
    2. In the immortalized mouse and primary cultured proliferative rat hepatocytes, treatment with OSM markedly increased mRNA and protein of claudin-2 together with formation of developed networks of TJ strands. PMID: 17434483
    3. OSM may not only play a role in the regulation of Sertoli cell proliferation and the initiation of spermatogenesis but may also play a role in the regulation of Leydig cell progenitor formation. PMID: 17996055
    4. Results indicate that osteosarcoma cells stably producing OSM do not develop resistance to this cytokine and thus could be a valuable new tool to study the anti-cancer effect of OSM in vivo. PMID: 19168167
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    LIF/OSM family
  • 组织特异性：
    Widely expressed. Expressed at higher levels in liver, skin and spleen.
  • 数据库链接：