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ALKBH5 Antibody

  • 货号：
    CSB-PA001611GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q6P6C2
  • 基因名：
    ALKBH5
  • 别名：
    ALKBH5 antibody; ABH5 antibody; OFOXD1RNA demethylase ALKBH5 antibody; EC 1.14.11.53 antibody; Alkylated DNA repair protein alkB homolog 5 antibody; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human ALKBH5
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
  • 基因功能参考文献：
    1. Overexpression of ALKBH5 in MIA-PaCa-2 and BxPC-3 cells, demonstrats that ALKBH5 could inhibit cell migration and invasion and ALKBH5 inhibits cell motility by demethylating and increasing expression of KCNK15- AS1. PMID: 30032148
    2. N(6)-Methyladenosine itself serves as a 'conformational marker', which induces different conformational outcomes in RNAs depending on sequence context. This critically impacts its interactions with several m6A-recognising proteins, including FTO and ALKBH5. PMID: 27156733
    3. ALKBH5 knockdown in breast cancer cells significantly decreased metastasis from breast to lungs in immunodeficient mice PMID: 27590511
    4. ALKBH5-FOXM1 pathway is critical for glioblastoma proliferation and tumorigenesis.ALKBH5 expression increases in glioblastoma stem-like cells and predicts poor survival in glioblastoma patients. PMID: 28344040
    5. HIF-dependent ALKBH5 expression mediates enrichment of BCSCs in the hypoxic tumor microenvironment PMID: 27001847
    6. The ALKBH5 gene may play a role in conferring risk of MDD in the Chinese population. PMID: 26047305
    7. Structures of human ALKBH5 demethylase reveal a unique binding mode for specific single-stranded N6-methyladenosine RNA demethylation. PMID: 24778178
    8. Modelling substrate into the active site of ALKBH5 reveals conserved residues important for recognition and demethylation mechanisms. PMID: 24489119
    9. findings provide a structural basis for understanding the substrate recognition specificity of Alkbh5 and offer a foundation for selective drug design against AlkB members PMID: 24616105
    10. ALKBH5 is a RNA demethylase and its action strongly suggests that the reversible methyladenosine modification has fundamental and broad functions in mammalian cells. PMID: 23177736
    11. ALKBH5 may have a role in the regulation of cellular responses to hypoxia as a class of HIF-transcriptional target gene PMID: 21264265

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  • 亚细胞定位：
    Nucleus speckle.
  • 蛋白家族：
    AlkB family
  • 组织特异性：
    Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
  • 数据库链接：

    HGNC: 25996

    OMIM: 613303

    KEGG: hsa:54890

    STRING: 9606.ENSP00000382091

    UniGene: Hs.744130