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  4. Recombinant Human RNA demethylase ALKBH5 (ALKBH5)

Recombinant Human RNA demethylase ALKBH5 (ALKBH5)

  • 货号：
    CSB-YP744062HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP744062HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP744062HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP744062HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ALKBH5
  • Uniprot No.：
  • 别名：
    ALKBH5; ABH5; OFOXD1RNA demethylase ALKBH5; EC 1.14.11.53; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-394
  • 氨基酸序列
    AAASGYTDL REKLKSMTSR DNYKAGSREA AAAAAAAVAA AAAAAAAAEP YPVSGAKRKY QEDSDPERSD YEEQQLQKEE EARKVKSGIR QMRLFSQDEC AKIEARIDEV VSRAEKGLYN EHTVDRAPLR NKYFFGEGYT YGAQLQKRGP GQERLYPPGD VDEIPEWVHQ LVIQKLVEHR VIPEGFVNSA VINDYQPGGC IVSHVDPIHI FERPIVSVSF FSDSALCFGC KFQFKPIRVS EPVLSLPVRR GSVTVLSGYA ADEITHCIRP QDIKERRAVI ILRKTRLDAP RLETKSLSSS VLPPSYASDR LSGNNRDPAL KPKRSHRKAD PDAAHRPRIL EMDKEENRRS VLLPTHRRRG SFSSENYWRK SYESSEDCSE AAGSPARKVK MRRH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
  • 基因功能参考文献：
    1. Overexpression of ALKBH5 in MIA-PaCa-2 and BxPC-3 cells, demonstrats that ALKBH5 could inhibit cell migration and invasion and ALKBH5 inhibits cell motility by demethylating and increasing expression of KCNK15- AS1. PMID: 30032148
    2. N(6)-Methyladenosine itself serves as a 'conformational marker', which induces different conformational outcomes in RNAs depending on sequence context. This critically impacts its interactions with several m6A-recognising proteins, including FTO and ALKBH5. PMID: 27156733
    3. ALKBH5 knockdown in breast cancer cells significantly decreased metastasis from breast to lungs in immunodeficient mice PMID: 27590511
    4. ALKBH5-FOXM1 pathway is critical for glioblastoma proliferation and tumorigenesis.ALKBH5 expression increases in glioblastoma stem-like cells and predicts poor survival in glioblastoma patients. PMID: 28344040
    5. HIF-dependent ALKBH5 expression mediates enrichment of BCSCs in the hypoxic tumor microenvironment PMID: 27001847
    6. The ALKBH5 gene may play a role in conferring risk of MDD in the Chinese population. PMID: 26047305
    7. Structures of human ALKBH5 demethylase reveal a unique binding mode for specific single-stranded N6-methyladenosine RNA demethylation. PMID: 24778178
    8. Modelling substrate into the active site of ALKBH5 reveals conserved residues important for recognition and demethylation mechanisms. PMID: 24489119
    9. findings provide a structural basis for understanding the substrate recognition specificity of Alkbh5 and offer a foundation for selective drug design against AlkB members PMID: 24616105
    10. ALKBH5 is a RNA demethylase and its action strongly suggests that the reversible methyladenosine modification has fundamental and broad functions in mammalian cells. PMID: 23177736
    11. ALKBH5 may have a role in the regulation of cellular responses to hypoxia as a class of HIF-transcriptional target gene PMID: 21264265

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  • 亚细胞定位：
    Nucleus speckle.
  • 蛋白家族：
    AlkB family
  • 组织特异性：
    Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
  • 数据库链接：

    HGNC: 25996

    OMIM: 613303

    KEGG: hsa:54890

    STRING: 9606.ENSP00000382091

    UniGene: Hs.744130