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CARM1 (Ab-228) Antibody

  • 货号：
    CSB-PA021890
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from A431 cells untreated or treated with EGF(200ng/ml, 5min), using CARM1(Ab-228) antibody(, Line 1 and 2) and CARM1(Phospho-Ser228) antibody(, Line 3 and 4).
    • Immunofluorescence staining of methanol-fixed HeLa cells using CARM1(Ab-228) antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CARM1 Polyclonal antibody
  • Uniprot No.：
    Q86X55
  • 基因名：
    CARM1
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
  • 基因功能参考文献：
    1. N-terminal EVH1 domain of CARM1 is necessary and sufficient for substrate recognition and is required for methylation of most CARM1 substrates. PMID: 28537268
    2. SKP2 promotes HCC progression and its nuclear functions of autophagy induction with CARM1 and AMPK, which may provide a potential target for HCC therapy. PMID: 29991055
    3. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3beta/beta-Catenin/cyclinD1 Signaling Pathway PMID: 28924379
    4. The CARM1-PKM2 axis serves as a metabolic reprogramming mechanism in tumorigenesis. PMID: 29058718
    5. Arginine (di)methylated human leukocyte antigen class I peptides, which are asymmetrically dimethylated, most likely by CARM1, are favorably presented by HLA-B*07. PMID: 27503676
    6. Estrogen receptor recruits steroid receptor coactivator-3 primary coactivator and secondary coactivators, p300/CBP and CARM1 to regulate genetic transcription. PMID: 28844863
    7. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
    8. Here, the crystal structures of human CARM1 with the S-adenosylmethione (SAM) mimic sinefungin and three different peptide sequences from histone H3 and PABP1 are presented, with both nonmethylated and singly methylated arginine residues exemplified. PMID: 26551522
    9. CARM1 associates with major nonsense-mediated mRNA decay factor UPF1 and promotes its occupancy on premature terminating codon-containing transcripts in spinal muscular atrophy. PMID: 26656492
    10. Monitoring of the CARM1-dependent production of monomethylated and dimethylated peptides over time by self-assembled monolayer and matrix-assisted laser desorption ionization mass spectrometry revealed that methylation by CARM1 is distributive. PMID: 26848779
    11. no obvious association of CARM1 isoform expression and clinical correlates in breast cancer PMID: 26030442
    12. disruption of CARM1 enhances the nuclear retention of mRNAs containing IRAlus PMID: 25792598
    13. Data indicate that coactivator-associated arginine methyltransferase 1 (CARM1) regulates neural differentiation through Nanog homeobox protein and microRNA miR92a. PMID: 25392304
    14. Like insulin, CARM1 overexpression increased CDK2 and CDK4 expression. In addition, CARM1 knockdown reduced the number of insulin-induced G2/M phase cells PMID: 25934150
    15. a noncoding variant in the CARM1-promoter functions as a regulator of gene transcription and homocysteine levels PMID: 25064859
    16. O-GlcNAcylation of CARM1 at its C-terminus is an important determinant for CARM1 substrate specificity. PMID: 25585345
    17. PRMT4 has a role in arginine methylation, which negatively regulates retinoblastoma tumor suppressor protein and promotes E2F-1 dissociation PMID: 25348716
    18. PBMCs from patients with ACS showed higher levels of CARM1 mRNA and protein expression.CARM1 regulated the transcription of IP-10, IL-8, and MCP-1. miR-15a modulated CARM1 expression through targeted binding to CARM1 3'-UTR. PMID: 24530761
    19. high-glucose-induced CARM1 expression increases RPE cell apoptosis via H3R17 asymmetric dimethylation PMID: 25072916
    20. CARM1 and PRMT1 are dysregulated in lung cancer; only CARM1 expression was found to be correlated with tumor differentiation and neither CARM1 nor PRMT1 expression was correlated with survival. PMID: 24211191
    21. Overexpression of PRMT4 represses miR-223 via methylation of RUNX1 and blocks myeloid differentiation. PMID: 24332853
    22. CARM1 overexpression was associated with poorly characterized clinicopathologic parameters and HER2 overexpression in invasive breast cancer. PMID: 23915145
    23. we identify BAF155 as a substrate for arginine methyltransferase CARM1. PMID: 24434208
    24. increased cytoplasmic CARM1, relative to nuclear levels, appear to be associated with self-identified African ethnicity and this result is being further investigated using quantified genetic ancestry measures. PMID: 23663560
    25. CARM1 could be a potential marker of luminal class subclassification and for target therapy, particularly in the ER-positive luminal-like subgroup. PMID: 23887673
    26. Arginine 42 of histone H3 (H3R42), is dimethylated by the methyltransferases coactivator arginine methyltransferase 1 (CARM1) and protein arginine methyltransferase 6 (PRMT6) in vitro and in vivo. PMID: 23980157
    27. Data show the transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. PMID: 23699174
    28. CARM1 represses replicative senescence by methylating HuR. PMID: 23837869
    29. Inhibition of CARM1 decreases PELP1 oncogenic activity. PMID: 23486015
    30. the miR-181c-CARM1 pathway has an important role in regulating the differentiation of human embryonic stem cells PMID: 23301034
    31. These findings reveal an important and so far unknown connection between PRMT4 and the chromatin remodeller Mi2 in c-Myb signalling. PMID: 23505388
    32. Findings indicate that expression of PRMT4/CARM1 and PRMT6 is deregulated in melanoma. PMID: 23265702
    33. found that CARM1 facilitates Sox2-mediated transactivation and directly methylates Sox2 at arginine 113 PMID: 22046437
    34. Signal transduction in cell death and survival involves a role for CARM1 in arginine methylation. PMID: 21445011
    35. A coactivator role of CARM1 in the dysregulation of beta-catenin activity in colorectal cancer cell growth and gene expression PMID: 21478268
    36. Findings suggest that coexpression of CARM1 and ERalpha may provide a better biomarker of well-differentiated breast cancer. PMID: 21282336
    37. CARM1 was particularly overexpressed in colorectal cancers while CARM1 expression was not prevalent in prostate and breast cancers. PMID: 20462455
    38. The physical interaction of CARM1 and PCAF is likely pivotal for the activation of PCAF in the downstream of CARM1 pathway for inducing myogenin under Tetradecanoylphorbol Acetate -induced differentiation. PMID: 20213728
    39. phosphorylation of CARM1 by PKA at a single serine is necessary and sufficient for direct binding to the unliganded hormone-binding domain of ERalpha, and the interaction is necessary for cAMP activation of ERalpha PMID: 20360387
    40. Methylates PAB1 and TARPP, suggesting a role in regulating transcription/translation. PMID: 11850402
    41. The CBP/p300 acetylase and the CARM1 methyltransferase can positively regulate the expression of estrogen-responsive genes, there is a crosstalk between lysine acetylation and arginine methylation on chromatin PMID: 12498683
    42. CARM1 plays a significant role in promoting the differentiation of early thymocyte progenitors, possibly through its direct action on TARPP. PMID: 15096520
    43. overexpression of CARM1 is involved in the development of prostate carcinoma PMID: 15221992
    44. results show that CARM1/PRMT4 is a novel transcriptional coactivator of NF-kappaB and functions as a promoter-specific regulator of NF-kappaB recruitment to chromatin PMID: 15616592
    45. CARM1 affects pre-mRNA splicing in an isoform-specific manner PMID: 15944154
    46. arginine methylation of CBP is required for IFN-gamma induction of MHC-II. A kinetic analysis shows that CIITA, CARM1, and H3-R17 methylation all precede CBP loading on the MHC-II promoter during IFN-gamma treatment. PMID: 16254053
    47. CARM1 has a role in signal-dependent control of gluconeogenic key enzyme genes PMID: 16330542
    48. CARM1 participates in NF-kappaB-mediated transcription through H3-R17 methylation and support a nonnuclear receptor-associated function for CARM1. PMID: 16497732
    49. CARM1 is essential for androgen receptor (AR) function and may play a role in prostate cancer progression. PMID: 16705743
    50. Results suggest that members of the SRC coactivator family, such as SRC-3, serve as substrates for the enzymatic coactivator coactivator-associated arginine methyltransferase 1 (CARM1). PMID: 16923966

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 组织特异性：
    Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
  • 数据库链接：

    HGNC: 23393

    OMIM: 603934

    KEGG: hsa:10498

    STRING: 9606.ENSP00000325690

    UniGene: Hs.323213