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  4. Recombinant Human Histone-arginine methyltransferase CARM1 (CARM1)

Recombinant Human Histone-arginine methyltransferase CARM1 (CARM1)

  • 货号：
    CSB-YP771462HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP771462HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP771462HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP771462HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP771462HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CARM1
  • Uniprot No.：
  • 别名：
    carm1; CARM1_HUMAN; Coactivator associated arginine methyltransferase 1; Coactivator-associated arginine methyltransferase 1; Histone arginine methyltransferase CARM 1; Histone arginine methyltransferase CARM1; Histone-arginine methyltransferase CARM1; PRMT 4; PRMT4; Protein arginine methyltransferase; Protein arginine N methyltransferase 4; Protein arginine N-methyltransferase 4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-608
  • 氨基酸序列
    AAAAAAVGP GAGGAGSAVP GGAGPCATVS VFPGARLLTI GDANGEIQRH AEQQALRLEV RAGPDSAGIA LYSHEDVCVF KCSVSRETEC SRVGKQSFII TLGCNSVLIQ FATPNDFCSF YNILKTCRGH TLERSVFSER TEESSAVQYF QFYGYLSQQQ NMMQDYVRTG TYQRAILQNH TDFKDKIVLD VGCGSGILSF FAAQAGARKI YAVEASTMAQ HAEVLVKSNN LTDRIVVIPG KVEEVSLPEQ VDIIISEPMG YMLFNERMLE SYLHAKKYLK PSGNMFPTIG DVHLAPFTDE QLYMEQFTKA NFWYQPSFHG VDLSALRGAA VDEYFRQPVV DTFDIRILMA KSVKYTVNFL EAKEGDLHRI EIPFKFHMLH SGLVHGLAFW FDVAFIGSIM TVWLSTAPTE PLTHWYQVRC LFQSPLFAKA GDTLSGTCLL IANKRQSYDI SIVAQVDQTG SKSSNLLDLK NPFFRYTGTT PSPPPGSHYT SPSENMWNTG STYNLSSGMA VAGMPTAYDL SSVIASGSSV GHNNLIPLAN TGIVNHTHSR MGSIMSTGIV QGSSGAQGSG GGSTSAHYAV NSQFTMGGPA ISMASPMSIP TNTMHYGS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
  • 基因功能参考文献：
    1. N-terminal EVH1 domain of CARM1 is necessary and sufficient for substrate recognition and is required for methylation of most CARM1 substrates. PMID: 28537268
    2. SKP2 promotes HCC progression and its nuclear functions of autophagy induction with CARM1 and AMPK, which may provide a potential target for HCC therapy. PMID: 29991055
    3. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3beta/beta-Catenin/cyclinD1 Signaling Pathway PMID: 28924379
    4. The CARM1-PKM2 axis serves as a metabolic reprogramming mechanism in tumorigenesis. PMID: 29058718
    5. Arginine (di)methylated human leukocyte antigen class I peptides, which are asymmetrically dimethylated, most likely by CARM1, are favorably presented by HLA-B*07. PMID: 27503676
    6. Estrogen receptor recruits steroid receptor coactivator-3 primary coactivator and secondary coactivators, p300/CBP and CARM1 to regulate genetic transcription. PMID: 28844863
    7. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
    8. Here, the crystal structures of human CARM1 with the S-adenosylmethione (SAM) mimic sinefungin and three different peptide sequences from histone H3 and PABP1 are presented, with both nonmethylated and singly methylated arginine residues exemplified. PMID: 26551522
    9. CARM1 associates with major nonsense-mediated mRNA decay factor UPF1 and promotes its occupancy on premature terminating codon-containing transcripts in spinal muscular atrophy. PMID: 26656492
    10. Monitoring of the CARM1-dependent production of monomethylated and dimethylated peptides over time by self-assembled monolayer and matrix-assisted laser desorption ionization mass spectrometry revealed that methylation by CARM1 is distributive. PMID: 26848779
    11. no obvious association of CARM1 isoform expression and clinical correlates in breast cancer PMID: 26030442
    12. disruption of CARM1 enhances the nuclear retention of mRNAs containing IRAlus PMID: 25792598
    13. Data indicate that coactivator-associated arginine methyltransferase 1 (CARM1) regulates neural differentiation through Nanog homeobox protein and microRNA miR92a. PMID: 25392304
    14. Like insulin, CARM1 overexpression increased CDK2 and CDK4 expression. In addition, CARM1 knockdown reduced the number of insulin-induced G2/M phase cells PMID: 25934150
    15. a noncoding variant in the CARM1-promoter functions as a regulator of gene transcription and homocysteine levels PMID: 25064859
    16. O-GlcNAcylation of CARM1 at its C-terminus is an important determinant for CARM1 substrate specificity. PMID: 25585345
    17. PRMT4 has a role in arginine methylation, which negatively regulates retinoblastoma tumor suppressor protein and promotes E2F-1 dissociation PMID: 25348716
    18. PBMCs from patients with ACS showed higher levels of CARM1 mRNA and protein expression.CARM1 regulated the transcription of IP-10, IL-8, and MCP-1. miR-15a modulated CARM1 expression through targeted binding to CARM1 3'-UTR. PMID: 24530761
    19. high-glucose-induced CARM1 expression increases RPE cell apoptosis via H3R17 asymmetric dimethylation PMID: 25072916
    20. CARM1 and PRMT1 are dysregulated in lung cancer; only CARM1 expression was found to be correlated with tumor differentiation and neither CARM1 nor PRMT1 expression was correlated with survival. PMID: 24211191
    21. Overexpression of PRMT4 represses miR-223 via methylation of RUNX1 and blocks myeloid differentiation. PMID: 24332853
    22. CARM1 overexpression was associated with poorly characterized clinicopathologic parameters and HER2 overexpression in invasive breast cancer. PMID: 23915145
    23. we identify BAF155 as a substrate for arginine methyltransferase CARM1. PMID: 24434208
    24. increased cytoplasmic CARM1, relative to nuclear levels, appear to be associated with self-identified African ethnicity and this result is being further investigated using quantified genetic ancestry measures. PMID: 23663560
    25. CARM1 could be a potential marker of luminal class subclassification and for target therapy, particularly in the ER-positive luminal-like subgroup. PMID: 23887673
    26. Arginine 42 of histone H3 (H3R42), is dimethylated by the methyltransferases coactivator arginine methyltransferase 1 (CARM1) and protein arginine methyltransferase 6 (PRMT6) in vitro and in vivo. PMID: 23980157
    27. Data show the transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. PMID: 23699174
    28. CARM1 represses replicative senescence by methylating HuR. PMID: 23837869
    29. Inhibition of CARM1 decreases PELP1 oncogenic activity. PMID: 23486015
    30. the miR-181c-CARM1 pathway has an important role in regulating the differentiation of human embryonic stem cells PMID: 23301034
    31. These findings reveal an important and so far unknown connection between PRMT4 and the chromatin remodeller Mi2 in c-Myb signalling. PMID: 23505388
    32. Findings indicate that expression of PRMT4/CARM1 and PRMT6 is deregulated in melanoma. PMID: 23265702
    33. found that CARM1 facilitates Sox2-mediated transactivation and directly methylates Sox2 at arginine 113 PMID: 22046437
    34. Signal transduction in cell death and survival involves a role for CARM1 in arginine methylation. PMID: 21445011
    35. A coactivator role of CARM1 in the dysregulation of beta-catenin activity in colorectal cancer cell growth and gene expression PMID: 21478268
    36. Findings suggest that coexpression of CARM1 and ERalpha may provide a better biomarker of well-differentiated breast cancer. PMID: 21282336
    37. CARM1 was particularly overexpressed in colorectal cancers while CARM1 expression was not prevalent in prostate and breast cancers. PMID: 20462455
    38. The physical interaction of CARM1 and PCAF is likely pivotal for the activation of PCAF in the downstream of CARM1 pathway for inducing myogenin under Tetradecanoylphorbol Acetate -induced differentiation. PMID: 20213728
    39. phosphorylation of CARM1 by PKA at a single serine is necessary and sufficient for direct binding to the unliganded hormone-binding domain of ERalpha, and the interaction is necessary for cAMP activation of ERalpha PMID: 20360387
    40. Methylates PAB1 and TARPP, suggesting a role in regulating transcription/translation. PMID: 11850402
    41. The CBP/p300 acetylase and the CARM1 methyltransferase can positively regulate the expression of estrogen-responsive genes, there is a crosstalk between lysine acetylation and arginine methylation on chromatin PMID: 12498683
    42. CARM1 plays a significant role in promoting the differentiation of early thymocyte progenitors, possibly through its direct action on TARPP. PMID: 15096520
    43. overexpression of CARM1 is involved in the development of prostate carcinoma PMID: 15221992
    44. results show that CARM1/PRMT4 is a novel transcriptional coactivator of NF-kappaB and functions as a promoter-specific regulator of NF-kappaB recruitment to chromatin PMID: 15616592
    45. CARM1 affects pre-mRNA splicing in an isoform-specific manner PMID: 15944154
    46. arginine methylation of CBP is required for IFN-gamma induction of MHC-II. A kinetic analysis shows that CIITA, CARM1, and H3-R17 methylation all precede CBP loading on the MHC-II promoter during IFN-gamma treatment. PMID: 16254053
    47. CARM1 has a role in signal-dependent control of gluconeogenic key enzyme genes PMID: 16330542
    48. CARM1 participates in NF-kappaB-mediated transcription through H3-R17 methylation and support a nonnuclear receptor-associated function for CARM1. PMID: 16497732
    49. CARM1 is essential for androgen receptor (AR) function and may play a role in prostate cancer progression. PMID: 16705743
    50. Results suggest that members of the SRC coactivator family, such as SRC-3, serve as substrates for the enzymatic coactivator coactivator-associated arginine methyltransferase 1 (CARM1). PMID: 16923966

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 组织特异性：
    Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
  • 数据库链接：

    HGNC: 23393

    OMIM: 603934

    KEGG: hsa:10498

    STRING: 9606.ENSP00000325690

    UniGene: Hs.323213