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CDK12 Antibody

  • 货号：
    CSB-PA882147LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
      All lanes: CDK12 antibody at 2µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 165, 164, 142 kDa
      Observed band size: 165 kDa
  • 其他：

产品详情

  • 产品描述：

    The CDK12 antibody is a polyclonal IgG antibody developed in a rabbit. The immunogen is the recombinant human CDK12 protein (1309-1490aa). The purity of this CDK12 antibody exceeds 95% using protein G purification. This CDK12 antibody is specifically reactive with human species. The reactivity of this OCLN antibody with the CDK12 protein has been confirmed by ELISA and WB assays.

    CDK12 is a kinase enzyme that regulates the expression of genes involved in various cellular processes, including transcription, DNA damage response and repair, and cell cycle regulation. Mutations in the CDK12 gene have been identified in a subset of tumors, including ovarian, breast, and prostate cancers.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CDK12 Polyclonal antibody
  • Uniprot No.：
    Q9NYV4
  • 基因名：
  • 别名：
    arginine/serine-rich antibody; Cdc2 related kinase arginine/serine rich antibody; CDC2 related protein kinase 7 antibody; Cdc2-related kinase antibody; CDC2-related protein kinase 7 antibody; Cdk12 antibody; CDK12_HUMAN antibody; Cell division cycle 2 related protein kinase 7 antibody; Cell division cycle 2-related protein kinase 7 antibody; Cell division protein kinase 12 antibody; CRK7 antibody; CRKR antibody; CrkRS antibody; Cyclin-dependent kinase 12 antibody; EC 2.7.11.22 antibody; hCDK12 antibody; kiaa0904 antibody; Pksc antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cyclin-dependent kinase 12 protein (1309-1490AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CDK12 Antibody (CSB-PA882147LA01HU)，的标记方式是Non-conjugated。对于CDK12 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA882147LB01HU CDK12 Antibody, HRP conjugated ELISA
    FITC CSB-PA882147LC01HU CDK12 Antibody, FITC conjugated
    Biotin CSB-PA882147LD01HU CDK12 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
  • 基因功能参考文献：
    1. Cyclin K expression positively correlates with cell proliferation; knockdown of cyclin K or its cognate kinase CDK12 prevents the assembly of prereplicative complex in the G1 cell cycle phase. PMID: 29760377
    2. CDK12 regulates prereplicative complex assembly to promote mammalian cell proliferation. PMID: 29760377
    3. CDK12 gene amplification can contribute to the pathogenesis of breast cancer. PMID: 28334900
    4. BRCA1 downregulation combined with CHK1 inhibition induced excessive amounts of DNA damage, resulting in an inability to complete the S-phase. Therefore, we suggest CHK1 inhibition as a strategy for targeting BRCA1- or CDK12-deficient tumors. PMID: 29025359
    5. In Ovarian Cancer patients CDK12 loss is consistently associated with a particular genomic instability pattern characterized by hundreds of tandem duplications PMID: 26787835
    6. Structures of CDK12/CycK complexes solved in the presence of AMP-PNP. PMID: 26597175
    7. Data show that most mutations prevent formation of the cyclin-dependent kinase 12 (Cdk12)/cyclin K (CycK) complex, rendering the kinase inactive. PMID: 25712099
    8. CDK12 affects RNA processing events in two distinct ways: Indirectly through generating factor-binding phospho-epitopes on the CTD of elongating RNAPII and directly through binding to specific factors PMID: 25429106
    9. CDK12 and CDK13 losses in HCT116 cells preferentially affect expression of DNA damage response. PMID: 25561469
    10. CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
    11. CDK12 has properties that should confirm interest in its use as a biomarker. PMID: 24240700
    12. many CDK12 mutations are an unrecognized cause of HR defects in ovarian cancers PMID: 24554720
    13. CDK12 interacts with cyclin K and is required to maintain embryonnic stem cell self-renewal. PMID: 22547058
    14. Cyclin K1 is the primary cyclin partner for CDK12/CrkRS and it is required for activation of CDK12/CrkRS to phosphorylate the C-terminal domain of RNA Pol II. PMID: 22988298
    15. through regulation of expression of DNA damage response genes, CycK/Cdk12 protects cells from genomic instability PMID: 22012619
    16. Data show that siRNA knockdown of hCDK12 in HeLa cells results in alterations in the CTD phosphorylation state. PMID: 20952539
    17. CRK7 modifies MAP kinases regulation and resistance to estrogen signaling inhibitors in breast cancers. PMID: 19651820

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  • 相关疾病：
    Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
  • 亚细胞定位：
    Nucleus. Nucleus speckle. Note=Colocalized with nuclear speckles throughout interphase.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 24224

    OMIM: 615514

    KEGG: hsa:51755

    STRING: 9606.ENSP00000398880

    UniGene: Hs.345028