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CELF2 Antibody

  • 货号：
    CSB-PA006217LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate
      All lanes: CELF2 antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 55, 53, 56 kDa
      Observed band size: 55 kDa
    • IHC image of CSB-PA006217LA01HU diluted at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA006217LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescent analysis of A549 cells using CSB-PA006217LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CELF2 Polyclonal antibody
  • Uniprot No.：
    O95319
  • 基因名：
    CELF2
  • 别名：
    Bruno-like protein 3 antibody; BRUNOL3 antibody; Cardiac elav type RNA binding protein antibody; CELF-2 antibody; celf2 antibody; CELF2_HUMAN antibody; CUG BP2 antibody; CUG triplet repeat RNA-binding protein 2 antibody; CUG-BP- and ETR-3-like factor 2 antibody; CUG-BP2 antibody; CUGBP Elav like family member 2 antibody; CUGBP Elav-like family member 2 antibody; CUGBP2 antibody; dJ323N1.1 antibody; elav type RNA binding protein antibody; ELAV-type RNA-binding protein 3 antibody; ETR 3 antibody; ETR-3 antibody; ETR3 antibody; HGNC:2550 antibody; hNAPOR antibody; NAPOR 2 antibody; NAPOR antibody; Neuroblastoma apoptosis-related RNA-binding protein antibody; Neuroplastoma apoptosis related RNA binding protein 3 antibody; RNA binding protein BRUNOL3 antibody; RNA-binding protein BRUNOL-3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human CUGBP Elav-like family member 2 protein (291-381AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CELF2 Antibody (CSB-PA006217LA01HU)，的标记方式是Non-conjugated。对于CELF2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA006217LB01HU CELF2 Antibody, HRP conjugated ELISA
    FITC CSB-PA006217LC01HU CELF2 Antibody, FITC conjugated
    Biotin CSB-PA006217LD01HU CELF2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA.
  • 基因功能参考文献：
    1. Overexpression of CELF2 could reverse miR-615-3p's oncogenic functions. PMID: 29501762
    2. CUG-BP2 binds to AU-rich motifs found in the COX-2 mRNA 3'-UTR. PMID: 28935965
    3. Study provides evidence for a generalized position- dependent activity of CELF2 in splicing that can be used to predict its consequence on alternative splicing in a cell-type independent manner. PMID: 27096301
    4. These data suggest an interplay between CELF2 and hnRNP C as the mechanistic basis for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331
    5. Haplotype-dependent allele-specific methylation of CELF2 gene is associated with neurological disorders. PMID: 27153397
    6. CUGBP2 expression at the messenger RNA (mRNA) level was 2.2-fold lower and could be associated with high chemoresistance and early dissemination of pancreatic cancer. PMID: 26691217
    7. the genetic variant rs3740194 in CELF2 gene might be a valuable predictor for NPC prognosis PMID: 26314850
    8. Data show miR95 expression level positively related to glioma grade and its downregulation affects proliferation, invasion and apoptosis by targeting CELF2. MiR95 is identified as a putative therapeutic target and CELF2 as a potential tumor suppressor. PMID: 26165303
    9. a widespread role for the JNK-CELF2 axis in controlling splicing during T-cell activation, including a specific role in propagating JNK signaling. PMID: 26443849
    10. The A allele of rs2242451 in CUGBP2 might decrease Alzheimer disease risk in the Chinese Han population. PMID: 25801238
    11. novel mechanisms for CELF2 regulation that may broadly impact CELF2 expression across diverse cell types. PMID: 25870297
    12. There was evidence of association for recently-reported late-onset Alzheimer's disease risk loci, including BIN1 and CLU and CUGBP2 with APOE. PMID: 21379329
    13. Alternative splicing of LEF1 exon 6 is regulated during pre-TCR signaling in thymic development and in response to activation of the JSL1 T-cell line and this is driven by the activity of CELF2. PMID: 21444716
    14. results indicate the occurrence of a mis-splicing event in myotonic dystrophy type 1 that is induced neither by a loss of muscleblind-like 1 (MBNL1) function nor by a gain of CUGBP1 PMID: 21439371
    15. BRUNOL3 appears to be an important factor for thymus development and is therefore a candidate gene for the thymus hypoplasia/aplasia seen in partial monosomy 10p patients. PMID: 12110949
    16. Determination of ETR-3 protein domains required for RNA splicing. PMID: 14973222
    17. data suggest that CUGBP2 is a critical regulator of the apoptotic response to genotoxic injury in breast cancer cells PMID: 15033780
    18. Lipopolysaccharide inhibition of CUGBP2 is a prostaglandin-dependent mechanism. PMID: 15358864
    19. Developmental upregulation of apoB mRNA editing from approximately 3% to 88% begins with decreased levels of inhibitory CUG binding protein 2 (CUGBP2) expression. PMID: 16920700
    20. CUGBP2 overrides HuR and suppresses COX-2 mRNA translation. PMID: 17383427
    21. Data demonstrate that cells expressing CUGBP2 variant 1 undergo apoptosis during mitosis, suggesting mitotic catastrophe. PMID: 18258790
    22. Demonstrate that CUGBP2 inhibits Mcl-1 expression by inhibiting Mcl-1 mRNA translation, resulting in driving the cells to apoptosis during the G(2) phase of the cell cycle. PMID: 18292181
    23. Data demonstrate that PGE(2) protects colon cancer cells from ionizing radiation-induced mitotic catastrophe in part through suppression of CUGBP2 expression. PMID: 18325984

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    CELF/BRUNOL family
  • 组织特异性：
    Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
  • 数据库链接：

    HGNC: 2550

    OMIM: 602538

    KEGG: hsa:10659

    STRING: 9606.ENSP00000389951

    UniGene: Hs.309288