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  4. Recombinant Human CUGBP Elav-like family member 2 (CELF2)

Recombinant Human CUGBP Elav-like family member 2 (CELF2)

  • 货号：
    CSB-YP006217HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006217HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006217HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006217HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006217HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CELF2
  • Uniprot No.：
  • 别名：
    Bruno-like protein 3; BRUNOL3; Cardiac elav type RNA binding protein; CELF-2; celf2; CELF2_HUMAN; CUG BP2; CUG triplet repeat RNA-binding protein 2; CUG-BP- and ETR-3-like factor 2; CUG-BP2; CUGBP Elav like family member 2; CUGBP Elav-like family member 2; CUGBP2; dJ323N1.1; elav type RNA binding protein; ELAV-type RNA-binding protein 3; ETR 3; ETR-3; ETR3; HGNC:2550; hNAPOR; NAPOR 2; NAPOR; Neuroblastoma apoptosis-related RNA-binding protein; Neuroplastoma apoptosis related RNA binding protein 3; RNA binding protein BRUNOL3 ; RNA-binding protein BRUNOL-3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-508
  • 氨基酸序列
    MRCPKSAVTM RNEELLLSNG TANKMNGALD HSDQPDPDAI KMFVGQIPRS WSEKELKELF EPYGAVYQIN VLRDRSQNPP QSKGCCFVTF YTRKAALEAQ NALHNIKTLP GMHHPIQMKP ADSEKSNAVE DRKLFIGMVS KKCNENDIRV MFSPFGQIEE CRILRGPDGL SRGCAFVTFS TRAMAQNAIK AMHQSQTMEG CSSPIVVKFA DTQKDKEQRR LQQQLAQQMQ QLNTATWGNL TGLGGLTPQY LALLQQATSS SNLGAFSGIQ QMAGMNALQL QNLATLAAAA AAAQTSATST NANPLSTTSS ALGALTSPVA ASTPNSTAGA AMNSLTSLGT LQGLAGATVG LNNINALAGM AALNGGLGAT GLTNGTAGTM DALTQAYSGI QQYAAAALPT LYSQSLLQQQ SAAGSQKEGP EGANLFIYHL PQEFGDQDIL QMFMPFGNVI SAKVFIDKQT NLSKCFGFVS YDNPVSAQAA IQAMNGFQIG MKRLKVQLKR SKNDSKPY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA.
  • 基因功能参考文献：
    1. Overexpression of CELF2 could reverse miR-615-3p's oncogenic functions. PMID: 29501762
    2. CUG-BP2 binds to AU-rich motifs found in the COX-2 mRNA 3'-UTR. PMID: 28935965
    3. Study provides evidence for a generalized position- dependent activity of CELF2 in splicing that can be used to predict its consequence on alternative splicing in a cell-type independent manner. PMID: 27096301
    4. These data suggest an interplay between CELF2 and hnRNP C as the mechanistic basis for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331
    5. Haplotype-dependent allele-specific methylation of CELF2 gene is associated with neurological disorders. PMID: 27153397
    6. CUGBP2 expression at the messenger RNA (mRNA) level was 2.2-fold lower and could be associated with high chemoresistance and early dissemination of pancreatic cancer. PMID: 26691217
    7. the genetic variant rs3740194 in CELF2 gene might be a valuable predictor for NPC prognosis PMID: 26314850
    8. Data show miR95 expression level positively related to glioma grade and its downregulation affects proliferation, invasion and apoptosis by targeting CELF2. MiR95 is identified as a putative therapeutic target and CELF2 as a potential tumor suppressor. PMID: 26165303
    9. a widespread role for the JNK-CELF2 axis in controlling splicing during T-cell activation, including a specific role in propagating JNK signaling. PMID: 26443849
    10. The A allele of rs2242451 in CUGBP2 might decrease Alzheimer disease risk in the Chinese Han population. PMID: 25801238
    11. novel mechanisms for CELF2 regulation that may broadly impact CELF2 expression across diverse cell types. PMID: 25870297
    12. There was evidence of association for recently-reported late-onset Alzheimer's disease risk loci, including BIN1 and CLU and CUGBP2 with APOE. PMID: 21379329
    13. Alternative splicing of LEF1 exon 6 is regulated during pre-TCR signaling in thymic development and in response to activation of the JSL1 T-cell line and this is driven by the activity of CELF2. PMID: 21444716
    14. results indicate the occurrence of a mis-splicing event in myotonic dystrophy type 1 that is induced neither by a loss of muscleblind-like 1 (MBNL1) function nor by a gain of CUGBP1 PMID: 21439371
    15. BRUNOL3 appears to be an important factor for thymus development and is therefore a candidate gene for the thymus hypoplasia/aplasia seen in partial monosomy 10p patients. PMID: 12110949
    16. Determination of ETR-3 protein domains required for RNA splicing. PMID: 14973222
    17. data suggest that CUGBP2 is a critical regulator of the apoptotic response to genotoxic injury in breast cancer cells PMID: 15033780
    18. Lipopolysaccharide inhibition of CUGBP2 is a prostaglandin-dependent mechanism. PMID: 15358864
    19. Developmental upregulation of apoB mRNA editing from approximately 3% to 88% begins with decreased levels of inhibitory CUG binding protein 2 (CUGBP2) expression. PMID: 16920700
    20. CUGBP2 overrides HuR and suppresses COX-2 mRNA translation. PMID: 17383427
    21. Data demonstrate that cells expressing CUGBP2 variant 1 undergo apoptosis during mitosis, suggesting mitotic catastrophe. PMID: 18258790
    22. Demonstrate that CUGBP2 inhibits Mcl-1 expression by inhibiting Mcl-1 mRNA translation, resulting in driving the cells to apoptosis during the G(2) phase of the cell cycle. PMID: 18292181
    23. Data demonstrate that PGE(2) protects colon cancer cells from ionizing radiation-induced mitotic catastrophe in part through suppression of CUGBP2 expression. PMID: 18325984

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    CELF/BRUNOL family
  • 组织特异性：
    Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
  • 数据库链接：

    HGNC: 2550

    OMIM: 602538

    KEGG: hsa:10659

    STRING: 9606.ENSP00000389951

    UniGene: Hs.309288