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CLEC4E Antibody

  • 货号：
    CSB-PA891553LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA891553LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA891553LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of U251 cells with CSB-PA891553LA01HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CLEC4E Polyclonal antibody
  • Uniprot No.：
    Q9ULY5
  • 基因名：
    CLEC4E
  • 别名：
    C type lectin domain family 4 member E antibody; C type lectin superfamily member 9 antibody; C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9 antibody; C-type lectin domain family 4 member E antibody; C-type lectin superfamily member 9 antibody; CLC4E_HUMAN antibody; CLEC 4E antibody; Clec4e antibody; CLECSF9 antibody; Macrophage inducible C type lectin antibody; Macrophage-inducible C-type lectin antibody; MINCLE antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human C-type lectin domain family 4 member E protein (41-219AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CLEC4E Antibody (CSB-PA891553LA01HU)，的标记方式是Non-conjugated。对于CLEC4E Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA891553LB01HU CLEC4E Antibody, HRP conjugated ELISA
    FITC CSB-PA891553LC01HU CLEC4E Antibody, FITC conjugated
    Biotin CSB-PA891553LD01HU CLEC4E Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue.
  • 基因功能参考文献：
    1. Mincle has the ability to survey mycolate-derived glycolipids from actinomycetes, distinguishing non-pathogenic (e.g. Rhodococcus spp.) and pathogenic (e.g. Mycobacterium tuberculosis) species on the basis of alpha-chain length. PMID: 27714279
    2. No association was detected with any of the SNPs analysed and susceptibility to tuberculosis. PMID: 27363694
    3. combination adjuvant systems demonstrate markedly different immune activation with age, with combined DC activation via Macrophage-inducible C-type lectin and TLR7/8 representing a novel approach to enhance the efficacy of early-life vaccines. PMID: 27793997
    4. We here show that Mincle gene expression was induced in alveolar macrophages and neutrophils in bronchoalveolar lavage fluids of mice and patients with pneumococcal pneumonia PMID: 27923071
    5. a nonredundant role for Clec4e in coordinating major biological pathways involved in atherosclerosis PMID: 27587433
    6. CLEC4E expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    7. The expression of Mincle increases significantly during the early period of Aspergillus fumigatus infection, while expression of eight corresponding cytokines changes. Mincle, as a pattern recognition receptor, may play a role in the early innate immune response of the corneal resistance against fungus. PMID: 28141595
    8. Induction of Mincle by Helicobacter pylori and consequent anti-inflammatory signaling denote a bacterial survival strategy. PMID: 26456705
    9. our findings identify mincle as a contributor to the inflammatory response after traumatic brain injury PMID: 25111533
    10. results suggest that cholesterol crystals are an endogenous ligand for hMincle and that they activate innate immune responses PMID: 26296894
    11. Data indicate that MINCLE receptor is able to mediate the response to trehalose-6,6-dimycolate (TDM) dependent on SYK kinase and CARD9 protein. PMID: 26202982
    12. mincle is a fungal receptor that can suppress antifungal immunity and, as such, is a potential therapeutic target. PMID: 24721577
    13. These results demonstrated that GroMM is a unique ligand for human Mincle that is not recognized by mouse Mincle. PMID: 24733387
    14. Results show the molecular mechanism of glycolipid recognition through C-type lectin receptors, which may provide clues to rational design for effective adjuvants. PMID: 24101491
    15. Macrophage-inducible C-type lectin is associated with anti-cyclic citrullinated peptide antibodies-positive rheumatoid arthritis in men. PMID: 22932191
    16. Mincle is a potentially critical player in human B cell responses. PMID: 22698596
    17. Data indicate an unusual pattern of reciprocal expression of Mincle on peripheral blood monocytes or neutrophils. PMID: 22641025
    18. silencing of renal DNaseI gene expression initiates a cascade of inflammatory signals including activation of Toll like receptors and Clec4e, leading to progression of both murine and human lupus nephritis PMID: 22479529
    19. Our data suggest that Mincle is induced in adipose tissue macrophages in obesity at least partly through the saturated fatty acid/TLR4/NF-kappaB pathway, thereby suggesting its pathophysiologic role in obesity-induced adipose tissue inflammation. PMID: 21282371
    20. progenitor-derived mast cells expressed the macrophage-inducible C-type lectin receptor Mincle, and exposure of these cells to M. sympodialis induced up-regulation of the mRNA expression of Mincle PMID: 20608913
    21. Several functional states mediating the interaction of Mincle and yeast at the surface of the macrophage. PMID: 18509109
    22. important for the initiation of an innate immune response to Candida albicans PMID: 19120481

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein. Cell projection, phagocytic cup.
  • 组织特异性：
    Expressed in monocytes and macrophages.
  • 数据库链接：

    HGNC: 14555

    OMIM: 609962

    KEGG: hsa:26253

    STRING: 9606.ENSP00000299663

    UniGene: Hs.236516