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  4. Recombinant Human C-type lectin domain family 4 member E (CLEC4E), partial

Recombinant Human C-type lectin domain family 4 member E (CLEC4E), partial

  • 中文名称：
    Recombinant Human C-type lectin domain family 4 member E(CLEC4E),partial,Yeast
  • 货号：
    CSB-YP891553HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human C-type lectin domain family 4 member E(CLEC4E),partial,Yeast
  • 货号：
    CSB-EP891553HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human C-type lectin domain family 4 member E(CLEC4E),partial,Yeast
  • 货号：
    CSB-EP891553HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human C-type lectin domain family 4 member E(CLEC4E),partial,Yeast
  • 货号：
    CSB-BP891553HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human C-type lectin domain family 4 member E(CLEC4E),partial,Yeast
  • 货号：
    CSB-MP891553HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CLEC4E
  • Uniprot No.：
  • 别名：
    C type lectin domain family 4 member E; C type lectin superfamily member 9; C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; CLC4E_HUMAN; CLEC 4E; Clec4e; CLECSF9; Macrophage inducible C type lectin; Macrophage-inducible C-type lectin; MINCLE
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue.
  • 基因功能参考文献：
    1. Mincle has the ability to survey mycolate-derived glycolipids from actinomycetes, distinguishing non-pathogenic (e.g. Rhodococcus spp.) and pathogenic (e.g. Mycobacterium tuberculosis) species on the basis of alpha-chain length. PMID: 27714279
    2. No association was detected with any of the SNPs analysed and susceptibility to tuberculosis. PMID: 27363694
    3. combination adjuvant systems demonstrate markedly different immune activation with age, with combined DC activation via Macrophage-inducible C-type lectin and TLR7/8 representing a novel approach to enhance the efficacy of early-life vaccines. PMID: 27793997
    4. We here show that Mincle gene expression was induced in alveolar macrophages and neutrophils in bronchoalveolar lavage fluids of mice and patients with pneumococcal pneumonia PMID: 27923071
    5. a nonredundant role for Clec4e in coordinating major biological pathways involved in atherosclerosis PMID: 27587433
    6. CLEC4E expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    7. The expression of Mincle increases significantly during the early period of Aspergillus fumigatus infection, while expression of eight corresponding cytokines changes. Mincle, as a pattern recognition receptor, may play a role in the early innate immune response of the corneal resistance against fungus. PMID: 28141595
    8. Induction of Mincle by Helicobacter pylori and consequent anti-inflammatory signaling denote a bacterial survival strategy. PMID: 26456705
    9. our findings identify mincle as a contributor to the inflammatory response after traumatic brain injury PMID: 25111533
    10. results suggest that cholesterol crystals are an endogenous ligand for hMincle and that they activate innate immune responses PMID: 26296894
    11. Data indicate that MINCLE receptor is able to mediate the response to trehalose-6,6-dimycolate (TDM) dependent on SYK kinase and CARD9 protein. PMID: 26202982
    12. mincle is a fungal receptor that can suppress antifungal immunity and, as such, is a potential therapeutic target. PMID: 24721577
    13. These results demonstrated that GroMM is a unique ligand for human Mincle that is not recognized by mouse Mincle. PMID: 24733387
    14. Results show the molecular mechanism of glycolipid recognition through C-type lectin receptors, which may provide clues to rational design for effective adjuvants. PMID: 24101491
    15. Macrophage-inducible C-type lectin is associated with anti-cyclic citrullinated peptide antibodies-positive rheumatoid arthritis in men. PMID: 22932191
    16. Mincle is a potentially critical player in human B cell responses. PMID: 22698596
    17. Data indicate an unusual pattern of reciprocal expression of Mincle on peripheral blood monocytes or neutrophils. PMID: 22641025
    18. silencing of renal DNaseI gene expression initiates a cascade of inflammatory signals including activation of Toll like receptors and Clec4e, leading to progression of both murine and human lupus nephritis PMID: 22479529
    19. Our data suggest that Mincle is induced in adipose tissue macrophages in obesity at least partly through the saturated fatty acid/TLR4/NF-kappaB pathway, thereby suggesting its pathophysiologic role in obesity-induced adipose tissue inflammation. PMID: 21282371
    20. progenitor-derived mast cells expressed the macrophage-inducible C-type lectin receptor Mincle, and exposure of these cells to M. sympodialis induced up-regulation of the mRNA expression of Mincle PMID: 20608913
    21. Several functional states mediating the interaction of Mincle and yeast at the surface of the macrophage. PMID: 18509109
    22. important for the initiation of an innate immune response to Candida albicans PMID: 19120481

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein. Cell projection, phagocytic cup.
  • 组织特异性：
    Expressed in monocytes and macrophages.
  • 数据库链接：

    HGNC: 14555

    OMIM: 609962

    KEGG: hsa:26253

    STRING: 9606.ENSP00000299663

    UniGene: Hs.236516