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PNMT Antibody

  • 货号：
    CSB-PA02939A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA02939A0Rb diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA02939A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PNMT Polyclonal antibody
  • Uniprot No.：
    P11086
  • 基因名：
    PNMT
  • 别名：
    Noradrenaline N-methyltransferase antibody; PENT antibody; Phenylethanolamine N-methyltransferase antibody; PNMT antibody; PNMT_HUMAN antibody; PNMTase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Phenylethanolamine N-methyltransferase protein (1-176AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PNMT Antibody (CSB-PA02939A0Rb)，的标记方式是Non-conjugated。对于PNMT Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA02939B0Rb PNMT Antibody, HRP conjugated ELISA
    FITC CSB-PA02939C0Rb PNMT Antibody, FITC conjugated
    Biotin CSB-PA02939D0Rb PNMT Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Converts noradrenaline to adrenaline.
  • 基因功能参考文献：
    1. PNMT underexpression is associated with malignant pheochromocytoma and paraganglioma. PMID: 27007161
    2. initial velocity studies on human PNMT in the absence and presence of product and dead end inhibitors. PMID: 24018397
    3. The 2-SNP AA haplotype in the PNMT promoter is associated with decreased risk of essential hypertension in Han Chinese. PMID: 21866188
    4. PNMT G-390A polymorphism is possibly associated with essential hypertension risk in male Chinese Han population. PMID: 19952402
    5. In Caucasians, phenylethanolamine N-methyltransferase single nucleotide polymorphisms are associated with the development of acute kidney injury, disease severity, and in-hospital mortality. PMID: 20090367
    6. PNMT variants in cis may interact with nuclear factors in trans to govern adrenergic activity. PMID: 20204374
    7. Human PNMT has been crystallized in complex with an inhibitor and the cofactor product S-adenosyl-L-homocysteine and diffraction data measured from a cryocooled crystal extend to a resolution of 2.3 A. PMID: 11807261
    8. pharmacologic and biochemical evidence of beta-carboline 2N-methyltransferase activity PMID: 11900856
    9. Association between the phenylethanolamine N-methyltransferase gene and multiple sclerosis. PMID: 11958827
    10. homozygosity/heterozygosity of the phenylethanolamine N-methyltransferase gene polymorphism was highly predictive of significant weight loss with sibutramine during the first 3 months of obesity treatment PMID: 12634439
    11. genetic variants of PNMT may play a role in the development of essential hypertension. PMID: 14553966
    12. Presence of local PNMT transcription in human heart after transplantation. PMID: 15848714
    13. disulfide-linked dimers are as active as the monomeric enzyme indicating that the crystal structure of the protein is a valid target for inhibitor design. PMID: 15893506
    14. Nicotinic stimuli appear to regulate expression of the epinephrine-synthesizing gene PNMT through a previously uncharacterized regulatory element. PMID: 15968085
    15. Results suggest that PNMT catalyzes transfer of methyl to ligand amines only when "anchor" interactions, such as those identified for the beta-hydroxyls of p-octopamine and cis-AT, are present. PMID: 16363801
    16. the differences in PNMT expression between normotensives and hypertensives are not determined by the polymorphisms in this gene, but rather by the interplay of gene expression regulators, which may vary among individuals PMID: 17645789
    17. We examined the correlation between reward dependence (RD) trait, and 5 polymorphisms in genes of norepinephrine pathways. We found that rs3764351 in PNMT was significantly associated with reward dependence trait. PMID: 18702937
    18. Haplotypes of the phenylethanolamine N-methyltransferase (PNMT), catechol-O-methyltransferase (COMT) have functionally important effects on alcohol-induced cardiovascular symptoms by affecting blood catecholamine levels. PMID: 18715275
    19. The crystal structures illustrate the adaptability of the PNMT substrate binding site in accepting multi-fused ring systems, such as substituted norbornene, as well as noradrenochrome, the oxidation product of noradrenaline. PMID: 19570037
    20. the reaction takes place via an SN2 mechanism with methyl transfer being rate-limiting PMID: 19733262
    21. Oncogenomic recombination hotspot around the PPP1R1B-STARD3-TCAP-PNMT-PERLD1-ERBB2-C17orf37-GRB7 amplicon at human chromosome 17q12 is closely linked to evolutionary recombination hotspot around the GSDML-GSDM locus. PMID: 15010812

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  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
  • 数据库链接：

    HGNC: 9160

    OMIM: 171190

    KEGG: hsa:5409

    STRING: 9606.ENSP00000269582

    UniGene: Hs.1892