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  4. Recombinant Human Phenylethanolamine N-methyltransferase (PNMT)

Recombinant Human Phenylethanolamine N-methyltransferase (PNMT)

  • 货号：
    CSB-YP018274HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018274HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018274HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018274HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018274HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PNMT
  • Uniprot No.：
  • 别名：
    Noradrenaline N-methyltransferase; PENT; Phenylethanolamine N-methyltransferase; PNMT; PNMT_HUMAN; PNMTase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-282
  • 氨基酸序列
    MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Converts noradrenaline to adrenaline.
  • 基因功能参考文献：
    1. PNMT underexpression is associated with malignant pheochromocytoma and paraganglioma. PMID: 27007161
    2. initial velocity studies on human PNMT in the absence and presence of product and dead end inhibitors. PMID: 24018397
    3. The 2-SNP AA haplotype in the PNMT promoter is associated with decreased risk of essential hypertension in Han Chinese. PMID: 21866188
    4. PNMT G-390A polymorphism is possibly associated with essential hypertension risk in male Chinese Han population. PMID: 19952402
    5. In Caucasians, phenylethanolamine N-methyltransferase single nucleotide polymorphisms are associated with the development of acute kidney injury, disease severity, and in-hospital mortality. PMID: 20090367
    6. PNMT variants in cis may interact with nuclear factors in trans to govern adrenergic activity. PMID: 20204374
    7. Human PNMT has been crystallized in complex with an inhibitor and the cofactor product S-adenosyl-L-homocysteine and diffraction data measured from a cryocooled crystal extend to a resolution of 2.3 A. PMID: 11807261
    8. pharmacologic and biochemical evidence of beta-carboline 2N-methyltransferase activity PMID: 11900856
    9. Association between the phenylethanolamine N-methyltransferase gene and multiple sclerosis. PMID: 11958827
    10. homozygosity/heterozygosity of the phenylethanolamine N-methyltransferase gene polymorphism was highly predictive of significant weight loss with sibutramine during the first 3 months of obesity treatment PMID: 12634439
    11. genetic variants of PNMT may play a role in the development of essential hypertension. PMID: 14553966
    12. Presence of local PNMT transcription in human heart after transplantation. PMID: 15848714
    13. disulfide-linked dimers are as active as the monomeric enzyme indicating that the crystal structure of the protein is a valid target for inhibitor design. PMID: 15893506
    14. Nicotinic stimuli appear to regulate expression of the epinephrine-synthesizing gene PNMT through a previously uncharacterized regulatory element. PMID: 15968085
    15. Results suggest that PNMT catalyzes transfer of methyl to ligand amines only when "anchor" interactions, such as those identified for the beta-hydroxyls of p-octopamine and cis-AT, are present. PMID: 16363801
    16. the differences in PNMT expression between normotensives and hypertensives are not determined by the polymorphisms in this gene, but rather by the interplay of gene expression regulators, which may vary among individuals PMID: 17645789
    17. We examined the correlation between reward dependence (RD) trait, and 5 polymorphisms in genes of norepinephrine pathways. We found that rs3764351 in PNMT was significantly associated with reward dependence trait. PMID: 18702937
    18. Haplotypes of the phenylethanolamine N-methyltransferase (PNMT), catechol-O-methyltransferase (COMT) have functionally important effects on alcohol-induced cardiovascular symptoms by affecting blood catecholamine levels. PMID: 18715275
    19. The crystal structures illustrate the adaptability of the PNMT substrate binding site in accepting multi-fused ring systems, such as substituted norbornene, as well as noradrenochrome, the oxidation product of noradrenaline. PMID: 19570037
    20. the reaction takes place via an SN2 mechanism with methyl transfer being rate-limiting PMID: 19733262
    21. Oncogenomic recombination hotspot around the PPP1R1B-STARD3-TCAP-PNMT-PERLD1-ERBB2-C17orf37-GRB7 amplicon at human chromosome 17q12 is closely linked to evolutionary recombination hotspot around the GSDML-GSDM locus. PMID: 15010812

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  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
  • 数据库链接：

    HGNC: 9160

    OMIM: 171190

    KEGG: hsa:5409

    STRING: 9606.ENSP00000269582

    UniGene: Hs.1892