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PPM1F Antibody

  • 货号：
    CSB-PA018493LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse liver tissue
      All lanes: PPM1F antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 50, 39 kDa
      Observed band size: 50 kDa
    • IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA018493LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PPM1F Polyclonal antibody
  • Uniprot No.：
    P49593
  • 基因名：
    PPM1F
  • 别名：
    Ca(2+)/calmodulin-dependent protein kinase phosphatase antibody; CaM-kinase phosphatase antibody; CAMKP antibody; CaMKPase antibody; FEM 2 antibody; hFEM-2 antibody; KIAA0015 antibody; Partner of PIX 2 antibody; POPX2 antibody; PP2C phosphatase antibody; Ppm1f antibody; PPM1F_HUMAN antibody; Protein fem-2 homolog antibody; Protein phosphatase 1F (PP2C domain containing) antibody; Protein phosphatase 1F antibody; Protein phosphatase Mg2+/Mn2+ dependent 1F antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Protein phosphatase 1F protein (1-76AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PPM1F Antibody (CSB-PA018493LA01HU)，的标记方式是Non-conjugated。对于PPM1F Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA018493LB01HU PPM1F Antibody, HRP conjugated ELISA
    FITC CSB-PA018493LC01HU PPM1F Antibody, FITC conjugated
    Biotin CSB-PA018493LD01HU PPM1F Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
  • 基因功能参考文献：
    1. PPM1F overexpression suppressed cell proliferation and invasion and counteracted the tumour-promoting role of miR-590, but PPM1F knockdown reversed these effects. PMID: 29473240
    2. POPX2 is a negative regulator of TAK1 signaling pathway and modulates apoptosis through the regulation of TAK1 activity. PMID: 28906490
    3. PPM1F could work downstream of alpha9-nAchR to promote nicotine-induced carcinogenic signals. Thus, PPM1F expression could be used for prognostic diagnosis, or inhibited as a potential strategy for cancer prevention and therapy. PMID: 27769050
    4. This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion. PMID: 27976581
    5. Overexpression of PPM1F is associated with metastasis of hepatocellular carcinoma. PMID: 26498692
    6. POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. PMID: 24338362
    7. POPX2 might regulate cell motility through its regulation of the MAPK1/3, leading to changes in the cytoskeleton and cell motility. PMID: 23621870
    8. hCaMKP activity is reversibly regulated by oxidation/reduction at Cys-359 PMID: 22743349
    9. FHOD1 and PPM1F (direct regulators of the actin cytoskeleton) were identified as novel targets of miR-200c. Expression levels of FHOD1 and PPM1F were inversely correlated with miR-200c level in breast cancer cell lines and breast cancer patient samples. PMID: 22144583
    10. Data identify a biochemical pathway through which POPX2 exerts its apparent cellular function: the regulation of activity of glycogen synthase kinase-3, which in turn modulates extracellular signal-regulated kinase and cell motility. PMID: 21656682
    11. The POPX2 phosphatase regulates cancer cell motility and invasiveness. PMID: 20016286
    12. The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2) PMID: 11864573
    13. PPM1F plays a key role in the regulation of CaMKII intracellularly PMID: 15140879

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  • 蛋白家族：
    PP2C family
  • 数据库链接：

    HGNC: 19388

    KEGG: hsa:9647

    STRING: 9606.ENSP00000263212

    UniGene: Hs.112728