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  4. Recombinant Human Protein phosphatase 1F (PPM1F)

Recombinant Human Protein phosphatase 1F (PPM1F)

  • 中文名称：
    人PPM1F重组蛋白
  • 货号：
    CSB-YP018493HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PPM1F重组蛋白
  • 货号：
    CSB-EP018493HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PPM1F重组蛋白
  • 货号：
    CSB-EP018493HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PPM1F重组蛋白
  • 货号：
    CSB-BP018493HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PPM1F重组蛋白
  • 货号：
    CSB-MP018493HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PPM1F
  • Uniprot No.：
  • 别名：
    Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CAMKP; CaMKPase; FEM 2; hFEM-2; KIAA0015; Partner of PIX 2; POPX2; PP2C phosphatase; Ppm1f; PPM1F_HUMAN; Protein fem-2 homolog; Protein phosphatase 1F (PP2C domain containing); Protein phosphatase 1F; Protein phosphatase Mg2+/Mn2+ dependent 1F
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-454
  • 氨基酸序列
    MSSGAPQKSS PMASGAEETP GFLDTLLQDF PALLNPEDPL PWKAPGTVLS QEEVEGELAE LAMGFLGSRK APPPLAAALA HEAVSQLLQT DLSEFRKLPR EEEEEEEDDD EEEKAPVTLL DAQSLAQSFF NRLWEVAGQW QKQVPLAARA SQRQWLVSIH AIRNTRRKME DRHVSLPSFN QLFGLSDPVN RAYFAVFDGH GGVDAARYAA VHVHTNAARQ PELPTDPEGA LREAFRRTDQ MFLRKAKRER LQSGTTGVCA LIAGATLHVA WLGDSQVILV QQGQVVKLME PHRPERQDEK ARIEALGGFV SHMDCWRVNG TLAVSRAIGD VFQKPYVSGE ADAASRALTG SEDYLLLACD GFFDVVPHQE VVGLVQSHLT RQQGSGLRVA EELVAAARER GSHDNITVMV VFLRDPQELL EGGNQGEGDP QAEGRRQDLP SSLPEPETQA PPRS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
  • 基因功能参考文献：
    1. PPM1F overexpression suppressed cell proliferation and invasion and counteracted the tumour-promoting role of miR-590, but PPM1F knockdown reversed these effects. PMID: 29473240
    2. POPX2 is a negative regulator of TAK1 signaling pathway and modulates apoptosis through the regulation of TAK1 activity. PMID: 28906490
    3. PPM1F could work downstream of alpha9-nAchR to promote nicotine-induced carcinogenic signals. Thus, PPM1F expression could be used for prognostic diagnosis, or inhibited as a potential strategy for cancer prevention and therapy. PMID: 27769050
    4. This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion. PMID: 27976581
    5. Overexpression of PPM1F is associated with metastasis of hepatocellular carcinoma. PMID: 26498692
    6. POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. PMID: 24338362
    7. POPX2 might regulate cell motility through its regulation of the MAPK1/3, leading to changes in the cytoskeleton and cell motility. PMID: 23621870
    8. hCaMKP activity is reversibly regulated by oxidation/reduction at Cys-359 PMID: 22743349
    9. FHOD1 and PPM1F (direct regulators of the actin cytoskeleton) were identified as novel targets of miR-200c. Expression levels of FHOD1 and PPM1F were inversely correlated with miR-200c level in breast cancer cell lines and breast cancer patient samples. PMID: 22144583
    10. Data identify a biochemical pathway through which POPX2 exerts its apparent cellular function: the regulation of activity of glycogen synthase kinase-3, which in turn modulates extracellular signal-regulated kinase and cell motility. PMID: 21656682
    11. The POPX2 phosphatase regulates cancer cell motility and invasiveness. PMID: 20016286
    12. The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2) PMID: 11864573
    13. PPM1F plays a key role in the regulation of CaMKII intracellularly PMID: 15140879

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  • 蛋白家族：
    PP2C family
  • 数据库链接：

    HGNC: 19388

    KEGG: hsa:9647

    STRING: 9606.ENSP00000263212

    UniGene: Hs.112728