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  4. TMEM192 Antibody

TMEM192 Antibody

  • 货号：
    CSB-PA023784LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
      All lanes: TMEM192 antibody at 3.2µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 31 kDa
      Observed band size: 31 kDa
    • IHC image of CSB-PA023784LA01HU diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA023784LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA023784LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating TMEM192 in Jurkat whole cell lysate
      Lane 1: Rabbit control IgG (1µg) instead of CSB-PA023784LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA023784LA01HU (6µg) + Jurkat whole cell lysate (500µg)
      Lane 3: Jurkat whole cell lysate (10µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TMEM192 Polyclonal antibody
  • Uniprot No.：
    Q8IY95
  • 基因名：
    TMEM192
  • 别名：
    TMEM192; Transmembrane protein 192
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Transmembrane protein 192 protein (193-271AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，TMEM192 Antibody (CSB-PA023784LA01HU)，的标记方式是Non-conjugated。对于TMEM192 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA023784LB01HU TMEM192 Antibody, HRP conjugated ELISA
    FITC CSB-PA023784LC01HU TMEM192 Antibody, FITC conjugated
    Biotin CSB-PA023784LD01HU TMEM192 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 基因功能参考文献：
    1. s found that transmembrane protein 192 (TMEM192) interacted with TIG1. s also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins. PMID: 27989102
    2. TMEM192 deficiency can induce autophagy in tumor cells, and can further activate apoptosis by the mitochondrial pathway through autophagy. PMID: 22736246
    3. TMEM192 was found to be strongly expressed in human kidney, liver, lung and pancreas tissue. The widespread tissue distribution could suggest an important role of TMEM192 for lysosomal function. PMID: 20370317
  • 亚细胞定位：
    Lysosome membrane; Multi-pass membrane protein. Late endosome.
  • 蛋白家族：
    TMEM192 family
  • 组织特异性：
    Strongly expressed in kidney, liver, lung and pancreas.
  • 数据库链接：

    HGNC: 26775

    KEGG: hsa:201931

    STRING: 9606.ENSP00000305069

    UniGene: Hs.704511