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TPR Antibody

  • 中文名称：
    TPR兔多克隆抗体
  • 货号：
    CSB-PA926943
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA926943(TPR Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    P12270
  • 基因名：
    TPR
  • 别名：
    Nucleoprotein TPR antibody; tpr antibody; TPR_HUMAN antibody; translocated promoter region (to activated MET oncogene) antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthetic peptide of Human TPR
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some s, plays a limited role in the regulation of nuclear protein export. Plays also a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases.
  • 基因功能参考文献：
    1. Fusion of TPR(2-142) protein to the MET intracellular domain strongly and selectively stabilizes the alphaG helix of the MET kinase domain.TPR has an ability to induce dimerization and activation of fusion kinases. PMID: 28528776
    2. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKAP95-dependent enrichment of TPR in the spindle microtubule area in metaphase, then later in the spindle midzone area. PMID: 28379780
    3. Tpr maintains a chromatin environment favourable for HIV replication. PMID: 25744187
    4. SLK interacts with Tpr and alpha-actinin-4 in cells, and these protein-protein interactions may control the subcellular localization and the biological activity of SLK. PMID: 26094769
    5. Protein kinase A phosphorylates the S2094 residue and that the site is hyperphosphorylated during mitosis. PMID: 24938596
    6. analysis of interactions between CRM1 and the nuclear pore protein Tpr and snurportin PMID: 24722547
    7. Tpr is a kinetochore-independent, rate-limiting factor required to mount and sustain a robust SAC response. PMID: 24344181
    8. Tpr import is mediated by the most abundant import receptor, KPNA2, which binds the bipartite NLS in Tpr with nanomolar affinity PMID: 23649804
    9. Tpr depletion increased p53 nuclear accumulation and facilitated autophagy. PMID: 23170199
    10. Exon 23 of the translocated promoter region gene was fused to exon 13 of FGFR1 resulting in 8p11 myeloproliferative syndrome. PMID: 22619110
    11. Perinuclear localization of Tpr at the nucleopore complex is crucial for regulating intron containing mRNA export. PMID: 22253824
    12. Depletion of Tpr by RNA interference triggers G0-G1 arrest and ultimately induces a senescent-like phenotype dependent on the presence of p53. PMID: 21811608
    13. Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway. PMID: 21613532
    14. Tpr has a role as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution PMID: 20407419
    15. results indicate that Tpr plays a critical role in the intranuclear dynamics of RNA pol II transcripts, including the processing, intranuclear transport and targeting, as well as their translocation through the NPC in mammalian cells PMID: 11952838
    16. model in which Tpr constitutes the central architectural element that forms the scaffold of the nuclear basket PMID: 15229283
    17. HSP mRNA export escapes stress inhibition via HSF1-mediated recruitment of the nuclear pore-associating protein TPR to HSP genes, functionally connecting the first and last nuclear steps of the gene expression PMID: 17897941
    18. candidate target gene TPR within 1q and 22q that are potentially important in the pathogenesis of intracranial pediatric ependymomas. PMID: 18663750
    19. Tpr regulates Mad1-Mad2 proteins are regulated during the cell cycle and mitotic spindle checkpoint signaling. PMID: 18981471

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  • 相关疾病：
    A chromosomal aberration involving TPR has been found in papillary thyroid carcinomas (PTCs). Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
  • 亚细胞定位：
    Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Nucleus envelope. Nucleus, nuclear pore complex. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    TPR family
  • 组织特异性：
    Expressed in esophagus, ovary, liver, skin, smooth muscles, cerebrum and fetal cerebellum (at protein level). Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
  • 数据库链接：

    HGNC: 12017

    OMIM: 189940

    KEGG: hsa:7175

    STRING: 9606.ENSP00000356448

    UniGene: Hs.279640