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ZNF224 Antibody

  • 货号：
    CSB-PA026592LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA026592LA01HU diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA026592LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ZNF224 Polyclonal antibody
  • Uniprot No.：
    Q9NZL3
  • 基因名：
    ZNF224
  • 别名：
    BMZF 2 antibody; BMZF-2 antibody; BMZF2 antibody; Bone marrow zinc finger 2 antibody; KOX22 antibody; Zinc finger 2; bone marrow antibody; Zinc finger protein 224 antibody; Zinc finger protein 233 antibody; Zinc finger protein 255 antibody; Zinc finger protein 27 (KOX 22) antibody; Zinc finger protein 27 antibody; Zinc finger protein KOX22 antibody; Zinc finger protein ZNF255 antibody; ZN224_HUMAN antibody; ZNF224 antibody; ZNF255 antibody; ZNF27 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Zinc finger protein 224 protein (477-607AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ZNF224 Antibody (CSB-PA026592LA01HU)，的标记方式是Non-conjugated。对于ZNF224 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA026592LB01HU ZNF224 Antibody, HRP conjugated ELISA
    FITC CSB-PA026592LC01HU ZNF224 Antibody, FITC conjugated
    Biotin CSB-PA026592LD01HU ZNF224 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
  • 基因功能参考文献：
    1. identify the receptor tyrosine kinase Axl as a novel target of ZNF224 transcriptional repression activity. PMID: 30176265
    2. The analyses using human breast ductal carcinoma tissues exhibited that the expression of ZNF224 and miR-663a was increased in cancer compared to non-cancer region. PMID: 27105517
    3. we showed that ZNF224 positively modulates cyclin D3 gene expression. Consistently, we observed that alteration of ZNF224 expression leads to defects in cell cycle control. All together, our results strongly suggest that in Chronic lymphocytic leukaemia (CLL)cells high expression level of ZNF224 can lead to inappropriate cell growth and apoptosis resistance, thus contributing to CLL progression PMID: 28040726
    4. Increasing information on the mechanism through which ZNF224 can operate could lead to the identification of agents capable of modulating ZNF224 function, thus potentially paving the way to new therapeutic strategies for treatment of cancer PMID: 28215224
    5. REVIEW: ZNF224 is a multifunctional protein and alternative splicing, sub-cellular compartmentalization and isoform-specific interactions may modulate its activity PMID: 21187159
    6. Coimmunoprecipitation and immunocytochemistry revealed that DEPDC1 interacted and colocalized with zinc finger transcription factor ZNF224, a known transcriptional repressor. PMID: 20587513
    7. We demonstrate that ZNF224, a Kruppel-like zinc finger transcription factor, is the repressor protein that specifically binds to the negative cis-element AldA-NRE and affects the AldA-NRE-mediated transcription PMID: 12527367
    8. Differential expression and cellular localization of ZNF224 and ZNF255, two isoforms of the Kruppel-like zinc-finger protein family. PMID: 17900823
    9. CIC silencer activity extends over 26 bp (-595/-569), which specifically bind a protein ZNF224 present in HepG2 cell nuclear extracts. PMID: 19505435
    10. ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270

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  • 亚细胞定位：
    Nucleus. Note=Colocalizes with DEPDC1A at the nucleus.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 组织特异性：
    Ubiquitous. Mainly expressed in fetal tissues.
  • 数据库链接：

    HGNC: 13017

    OMIM: 194555

    KEGG: hsa:7767

    STRING: 9606.ENSP00000337368

    UniGene: Hs.585869