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  4. Recombinant Human Zinc finger protein 224 (ZNF224)

Recombinant Human Zinc finger protein 224 (ZNF224)

  • 货号：
    CSB-YP026592HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026592HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026592HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026592HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026592HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ZNF224
  • Uniprot No.：
  • 别名：
    BMZF 2; BMZF-2; BMZF2; Bone marrow zinc finger 2; KOX22; Zinc finger 2; bone marrow; Zinc finger protein 224; Zinc finger protein 233; Zinc finger protein 255; Zinc finger protein 27 (KOX 22); Zinc finger protein 27; Zinc finger protein KOX22; Zinc finger protein ZNF255; ZN224_HUMAN; ZNF224; ZNF255; ZNF27
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-707
  • 氨基酸序列
    MTTFKEAMTF KDVAVVFTEE ELGLLDLAQR KLYRDVMLEN FRNLLSVGHQ AFHRDTFHFL REEKIWMMKT AIQREGNSGD KIQTEMETVS EAGTHQEWSF QQIWEKIASD LTRSQDLMIN SSQFSKEGDF PCQTEAGLSV IHTRQKSSQG NGYKPSFSDV SHFDFHQQLH SGEKSHTCDE CGKNFCYISA LRIHQRVHMG EKCYKCDVCG KEFSQSSHLQ THQRVHTGEK PFKCVECGKG FSRRSALNVH HKLHTGEKPY NCEECGKAFI HDSQLQEHQR IHTGEKPFKC DICGKSFCGR SRLNRHSMVH TAEKPFRCDT CDKSFRQRSA LNSHRMIHTG EKPYKCEECG KGFICRRDLY THHMVHTGEK PYNCKECGKS FRWASCLLKH QRVHSGEKPF KCEECGKGFY TNSQCYSHQR SHSGEKPYKC VECGKGYKRR LDLDFHQRVH TGEKLYNCKE CGKSFSRAPC LLKHERLHSG EKPFQCEECG KRFTQNSHLH SHQRVHTGEK PYKCEKCGKG YNSKFNLDMH QKVHTGERPY NCKECGKSFG WASCLLKHQR LHSGEKPFKC EECGKRFTQN SQLHSHQRVH TGEKPYKCDE CGKGFSWSST RLTHQRRHSR ETPLKCEQHG KNIVQNSFSK VQEKVHSVEK PYKCEDCGKG YNRRLNLDMH QRVHMGEKTW KCRECDMCFS QASSLRLHQN VHVGEKP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
  • 基因功能参考文献：
    1. identify the receptor tyrosine kinase Axl as a novel target of ZNF224 transcriptional repression activity. PMID: 30176265
    2. The analyses using human breast ductal carcinoma tissues exhibited that the expression of ZNF224 and miR-663a was increased in cancer compared to non-cancer region. PMID: 27105517
    3. we showed that ZNF224 positively modulates cyclin D3 gene expression. Consistently, we observed that alteration of ZNF224 expression leads to defects in cell cycle control. All together, our results strongly suggest that in Chronic lymphocytic leukaemia (CLL)cells high expression level of ZNF224 can lead to inappropriate cell growth and apoptosis resistance, thus contributing to CLL progression PMID: 28040726
    4. Increasing information on the mechanism through which ZNF224 can operate could lead to the identification of agents capable of modulating ZNF224 function, thus potentially paving the way to new therapeutic strategies for treatment of cancer PMID: 28215224
    5. REVIEW: ZNF224 is a multifunctional protein and alternative splicing, sub-cellular compartmentalization and isoform-specific interactions may modulate its activity PMID: 21187159
    6. Coimmunoprecipitation and immunocytochemistry revealed that DEPDC1 interacted and colocalized with zinc finger transcription factor ZNF224, a known transcriptional repressor. PMID: 20587513
    7. We demonstrate that ZNF224, a Kruppel-like zinc finger transcription factor, is the repressor protein that specifically binds to the negative cis-element AldA-NRE and affects the AldA-NRE-mediated transcription PMID: 12527367
    8. Differential expression and cellular localization of ZNF224 and ZNF255, two isoforms of the Kruppel-like zinc-finger protein family. PMID: 17900823
    9. CIC silencer activity extends over 26 bp (-595/-569), which specifically bind a protein ZNF224 present in HepG2 cell nuclear extracts. PMID: 19505435
    10. ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270

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  • 亚细胞定位：
    Nucleus. Note=Colocalizes with DEPDC1A at the nucleus.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 组织特异性：
    Ubiquitous. Mainly expressed in fetal tissues.
  • 数据库链接：

    HGNC: 13017

    OMIM: 194555

    KEGG: hsa:7767

    STRING: 9606.ENSP00000337368

    UniGene: Hs.585869