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KDM1B Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA958367A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate
      All lanes: KDM1B antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 93, 66, 20 kDa
      Observed band size: 90-95, 17-28 kDa
  • 其他：

产品详情

  • Uniprot No.：
    Q8NB78
  • 基因名：
    KDM1B
  • 别名：
    Lysine-specific histone demethylase 1B (EC 1.-.-.-) (Flavin-containing amine oxidase domain-containing protein 1) (Lysine-specific histone demethylase 2), KDM1B, AOF1 C6orf193 LSD2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human KDM1B
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    15F3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
  • 基因功能参考文献：
    1. These results suggest that LSD2 achieves a promoting effect on small cell lung cancer by indirectly regulating TFPI2 expression through the mediation of DNMT3B expression or through the regulation of the demethylation of H3K4me1 in the promoter region of the TFPI2 gene. PMID: 29845195
    2. This review focuses on published small-molecule inhibitors targeted at the two flavin adenine dinucleotide-dependent lysine demethylases, lysine-specific demethylases 1 and 2, and how the inhibitors interact with the tertiary structures of the enzymes. PMID: 28277979
    3. These results demonstrate an important role for LSD2 in regulation of DNA methylation and gene silencing in breast cancer. PMID: 24924415
    4. Histone demethylase LSD2 acts as an E3 ubiquitin ligase and inhibits cancer cell growth through promoting proteasomal degradation of OGT. PMID: 25773598
    5. Using transcriptome and chromatin immunoprecipitation-sequencing analyses, the study revealed that LSD2 represses the genes involved in lipid influx and metabolism through demethylation of histone H3K4. PMID: 25624347
    6. regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 PMID: 25036127
    7. The zinc finger-SWIRM-oxidase domains is required for KDM1B demethylase activity and the binding of FAD. PMID: 23266887
    8. Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation. PMID: 20670891

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 数据库链接：

    HGNC: 21577

    OMIM: 613081

    KEGG: hsa:221656

    STRING: 9606.ENSP00000297792

    UniGene: Hs.709336