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  4. FBP1 Recombinant Monoclonal Antibody

FBP1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA787345A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA787345A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    FBP1 catalyzes the hydrolysis of fructose-1,6-bisphosphate (F-1,6-BP) to fructose-6-phosphate and inorganic phosphate, which is the rate-limiting enzyme in glycolysis. F-1,6-BP is an allosteric activator of Pyruvate Kinase Type M2 (PKM2), a key enzyme in glycolysis. It's also a crucial tumor suppressor in human cancers. FBP1 expression is thought to be a possible prognostic factor for cancers such as gastric cancer, breast cancer, and lung cancer. FBP1 expression is reduced, which leads to an increase in glycolysis and the amount of cancer stem cells (CSCs). Furthermore, in addition to its role in glycolysis inhibition, overexpressed FBP1 has been shown to limit tumor growth and migration in breast cancer and renal cell carcinoma via interacting with the hypoxia-inducible factor (HIF) domain in some studies.

    The preparation of the recombinant FBP1 antibody involves the mammalian cell lines expression of plasma vectors containing FBP1 antibody genes. B cells isolated from immunized animals' blood were treated to obtain RNA, which underwent reverse transcription to yield DNA genes. Antibody genes were sequenced and screened from the DNA. After transient expression, cell supernatant was collected and then purified using Affinity-chromatography to obtain the recombinant FBP1 antibody. This recombinant FBP1 antibody is recommended to use in the FBP1 for the detection of FBP1 protein from Human.

  • Uniprot No.：
    P09467
  • 基因名：
  • 别名：
    Fructose-1,6-bisphosphatase 1 (FBPase 1) (EC 3.1.3.11) (D-fructose-1,6-bisphosphate 1-phosphohydrolase 1) (Liver FBPase), FBP1, FBP
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human FBP1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4G3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
  • 基因功能参考文献：
    1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
    2. Low FBP1 expression is associated with metastasis in cholangiocarcinoma. PMID: 30193944
    3. CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. PMID: 29678579
    4. Homozygous Alu element insertion in the FBP1 gene is associated with Fructose-1,6-bisphosphatase deficiency. PMID: 28599390
    5. decreased levels of FBP1 may be used as a predictor for poor prognosis of cervical cancer patients PMID: 28990097
    6. Study identified for the first time that HNF4alpha and C/EBPalpha are important transcriptional regulators for FBP1 expression in human hepatoma HepG2 cells. PMID: 29566023
    7. FBP1 mutation was associated with fructose-1,6-bisphosphatase deficiency PMID: 29016355
    8. Results identified FBP1 as a promising acute liver failure (ALF) biomarker among energy metabolism-related proteins. It may serve as a short-term prognosis indicator for ALF, with higher serum level of FBP1 correlated with higher ALF-related mortality in human studies. PMID: 28336726
    9. Here, the first crystal structure of human liver FBPase in the R-state conformation is presented, determined at a resolution of 2.2 A in a tetragonal setting that exhibits an unusual arrangement of noncrystallographic symmetry (NCS) elements. PMID: 27841754
    10. Studied association of fructose 1,6-bisphosphatase 1 (FBP1) expression with fluorine 18 ((18)F) fluorodeoxyglucose (FDG) accumulation in patients with hepatocellular carcinoma. Found that in patients with HCC, both 18F FDG accumulation and tumor grade (from differentiated to undifferentiated) were inversely correlated with the expression of FBP1. PMID: 28387640
    11. These findings indicate that FBP1 appears to be a tumor suppressor in hepatocellular carcinoma (HCC). Strategies to restore the levels and activities of FBP1 might be developed to treat patients with HCC. PMID: 27742690
    12. FBP1 underexpression is associated with Tumor Progression in Hepatocellular Carcinoma. PMID: 27197151
    13. fructose-1,6-bisphosphatase 1 facilitated co-action between Bcl-2 and Beclin 1, which may be important in the mechanism of fructose-1,6-bisphosphatase 1-mediated mitophagy inhibition. In summary, loss of mitophagy by fructose-1,6-bisphosphatase 1-mediated repression promotes apoptosis in breast cancer PMID: 28653874
    14. Downregulation of FBP1 promotes gastric cancer metastasis by facilitating EMT and acts as a potential prognostic factor and therapeutic target in gastric cancer. PMID: 27978536
    15. we show that EV71 viral proteinase 2A is capable of cleaving far upstream element-binding protein 1 (FBP1), a positive internal ribosome entry sitet rans-acting factor that directly binds to the EV71 5' UTR linker region to promote viral IRES-driven translation PMID: 27780225
    16. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery PMID: 27079415
    17. Elevated FBP1 is a critical modulator in breast cancer progression by altering glucose metabolism and the activity of Wnt/beta-catenin pathway. PMID: 27780144
    18. identified Zinc finger E-box-binding homeobox 1 (ZEB1) bond to FBP1 promoter to enhance DNA methylation in lung cancer cells. Our findings indicate that the down-regulation of FBP1 is a critical oncogenic event in lung cancer progression PMID: 26546081
    19. Twelve different mutations were identified in 22 alleles: one missense mutation c.472C > T, one point deletion c.48del, one point duplication c.865dupA, one deletion-insertion, and two splice mutations (c.427-1del and c.825 + 1G > A). PMID: 25601412
    20. FBP1 expression, which is closely correlated with c-Myc expression, is an independent prognostic factor and promotes nasopharyngeal carcinoma progression. PMID: 26469968
    21. NPM1 promotes aerobic glycolysis and tumor progression in patients with pancreatic cancer by inhibiting FBP1 PMID: 26068981
    22. There was a negative correlation with the level of FBP1 and recurrence of the lung cancer PMID: 25844935
    23. the gluconeogenic enzyme fructose-1,6-bisphosphatase 1 (FBP1) is uniformly depleted in over six hundred clear cell renal cell carcinoma tumours examined. PMID: 25043030
    24. Ca(2+) affects conformation of the catalytic loop 52-72 of muscle FBPase and inhibits its activity by competing with activatory divalent cations, e.g. Mg(2+) and Zn(2+). PMID: 24146906
    25. Case Reports: present reliable diagnostic system to verify an FBPase deficiency and find the genetic aberration. PMID: 20151204
    26. A novel missense mutation (c.841G>A) in the FBP1 gene seems to be prevalent in Pakistani-Indian patients with fructose-1,6-bisphosphatase deficiency. PMID: 23881342
    27. A homozygous c.658delT mutation was detected at exon 5 of the FBP1 gene in the two siblings with FBPase deficiency. PMID: 24007283
    28. LSD1 regulates transcription activation of two gluconeogenic genes, FBP1 and G6Pase. PMID: 23755305
    29. study indicates that the loss of FBP1 is a critical oncogenic event in epithelial-mesenchymal transition and basal-like breast cancer PMID: 23453623
    30. The Identification of FBPase interacting partners with mass spectrometry reveals a set of nuclear proteins involved in cell cycle regulation, mRNA processing and in stabilization of genomic DNA structure. PMID: 22057438
    31. This is the first study to identify liver FBPase as a previously unknown regulator of appetite and adiposity and describes a novel process by which the liver participates in body weight regulation. PMID: 22517657
    32. AMP binding pattern of the muscle isozyme of fructose-1,6-bisphosphatase is quite similar to that of the liver isozyme and the T conformations of the two isozymes are nearly the same PMID: 22120740
    33. epigenetic inactivation of FBP1 is also common in human liver and colon cancer. FBP1 appears to be a functional tumor suppressor involved in the liver and colon carcinogenesis PMID: 22039417
    34. Novel compound heterozygous mutations in the fructose-1,6-bisphosphatase gene cause hypoglycemia and lactic acidosis. PMID: 20096900
    35. first report on mutations in patients with FBP deficiency of Arab ethnicity,two novel mutations in the FBP1 gene encode for a duplication of two amino acids and a truncation in the FBP1 protein in these families. PMID: 19259699
    36. Data show that NF-kappaB functions downstream of Ras to promote epigenetic downregulation of FBP1. PMID: 19881551
    37. Data show that EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine. PMID: 19505453
    38. liver fructose-1,6-bisphosphatase coupled with phosphofructokinase (PFK) plays a crucial role in the metabolism of pancreatic islet cells PMID: 15965961
    39. Upregulation of FBPase in pancreatic islet beta-cells in states of lipid oversupply and type 2 diabetes, contributes to insulin secretory dysfunction. PMID: 18375435
    40. Human FBP1 was found to regulate mouse endogenous glucose production and whole body glucose homeostasis in a liver-specific transgenic model. PMID: 18780768
    41. this is the first experimental evidence confirming that the KKKGK motif can act as a functional NLS fructose 1,6-bisphosphatase . PMID: 19626708

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  • 相关疾病：
    Fructose-1,6-bisphosphatase deficiency (FBP1D)
  • 蛋白家族：
    FBPase class 1 family
  • 组织特异性：
    Expressed in pancreatic islets.
  • 数据库链接：

    HGNC: 3606

    OMIM: 229700

    KEGG: hsa:2203

    STRING: 9606.ENSP00000364475

    UniGene: Hs.494496