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  4. Recombinant Human Fructose-1,6-bisphosphatase 1 (FBP1)

Recombinant Human Fructose-1,6-bisphosphatase 1 (FBP1)

  • 货号：
    CSB-EP008459HUb1
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBP1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    41.7 kDa
  • 表达区域：
    2-338aa
  • 氨基酸序列
    ADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
  • 基因功能参考文献：
    1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
    2. Low FBP1 expression is associated with metastasis in cholangiocarcinoma. PMID: 30193944
    3. CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. PMID: 29678579
    4. Homozygous Alu element insertion in the FBP1 gene is associated with Fructose-1,6-bisphosphatase deficiency. PMID: 28599390
    5. decreased levels of FBP1 may be used as a predictor for poor prognosis of cervical cancer patients PMID: 28990097
    6. Study identified for the first time that HNF4alpha and C/EBPalpha are important transcriptional regulators for FBP1 expression in human hepatoma HepG2 cells. PMID: 29566023
    7. FBP1 mutation was associated with fructose-1,6-bisphosphatase deficiency PMID: 29016355
    8. Results identified FBP1 as a promising acute liver failure (ALF) biomarker among energy metabolism-related proteins. It may serve as a short-term prognosis indicator for ALF, with higher serum level of FBP1 correlated with higher ALF-related mortality in human studies. PMID: 28336726
    9. Here, the first crystal structure of human liver FBPase in the R-state conformation is presented, determined at a resolution of 2.2 A in a tetragonal setting that exhibits an unusual arrangement of noncrystallographic symmetry (NCS) elements. PMID: 27841754
    10. Studied association of fructose 1,6-bisphosphatase 1 (FBP1) expression with fluorine 18 ((18)F) fluorodeoxyglucose (FDG) accumulation in patients with hepatocellular carcinoma. Found that in patients with HCC, both 18F FDG accumulation and tumor grade (from differentiated to undifferentiated) were inversely correlated with the expression of FBP1. PMID: 28387640
    11. These findings indicate that FBP1 appears to be a tumor suppressor in hepatocellular carcinoma (HCC). Strategies to restore the levels and activities of FBP1 might be developed to treat patients with HCC. PMID: 27742690
    12. FBP1 underexpression is associated with Tumor Progression in Hepatocellular Carcinoma. PMID: 27197151
    13. fructose-1,6-bisphosphatase 1 facilitated co-action between Bcl-2 and Beclin 1, which may be important in the mechanism of fructose-1,6-bisphosphatase 1-mediated mitophagy inhibition. In summary, loss of mitophagy by fructose-1,6-bisphosphatase 1-mediated repression promotes apoptosis in breast cancer PMID: 28653874
    14. Downregulation of FBP1 promotes gastric cancer metastasis by facilitating EMT and acts as a potential prognostic factor and therapeutic target in gastric cancer. PMID: 27978536
    15. we show that EV71 viral proteinase 2A is capable of cleaving far upstream element-binding protein 1 (FBP1), a positive internal ribosome entry sitet rans-acting factor that directly binds to the EV71 5' UTR linker region to promote viral IRES-driven translation PMID: 27780225
    16. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery PMID: 27079415
    17. Elevated FBP1 is a critical modulator in breast cancer progression by altering glucose metabolism and the activity of Wnt/beta-catenin pathway. PMID: 27780144
    18. identified Zinc finger E-box-binding homeobox 1 (ZEB1) bond to FBP1 promoter to enhance DNA methylation in lung cancer cells. Our findings indicate that the down-regulation of FBP1 is a critical oncogenic event in lung cancer progression PMID: 26546081
    19. Twelve different mutations were identified in 22 alleles: one missense mutation c.472C > T, one point deletion c.48del, one point duplication c.865dupA, one deletion-insertion, and two splice mutations (c.427-1del and c.825 + 1G > A). PMID: 25601412
    20. FBP1 expression, which is closely correlated with c-Myc expression, is an independent prognostic factor and promotes nasopharyngeal carcinoma progression. PMID: 26469968
    21. NPM1 promotes aerobic glycolysis and tumor progression in patients with pancreatic cancer by inhibiting FBP1 PMID: 26068981
    22. There was a negative correlation with the level of FBP1 and recurrence of the lung cancer PMID: 25844935
    23. the gluconeogenic enzyme fructose-1,6-bisphosphatase 1 (FBP1) is uniformly depleted in over six hundred clear cell renal cell carcinoma tumours examined. PMID: 25043030
    24. Ca(2+) affects conformation of the catalytic loop 52-72 of muscle FBPase and inhibits its activity by competing with activatory divalent cations, e.g. Mg(2+) and Zn(2+). PMID: 24146906
    25. Case Reports: present reliable diagnostic system to verify an FBPase deficiency and find the genetic aberration. PMID: 20151204
    26. A novel missense mutation (c.841G>A) in the FBP1 gene seems to be prevalent in Pakistani-Indian patients with fructose-1,6-bisphosphatase deficiency. PMID: 23881342
    27. A homozygous c.658delT mutation was detected at exon 5 of the FBP1 gene in the two siblings with FBPase deficiency. PMID: 24007283
    28. LSD1 regulates transcription activation of two gluconeogenic genes, FBP1 and G6Pase. PMID: 23755305
    29. study indicates that the loss of FBP1 is a critical oncogenic event in epithelial-mesenchymal transition and basal-like breast cancer PMID: 23453623
    30. The Identification of FBPase interacting partners with mass spectrometry reveals a set of nuclear proteins involved in cell cycle regulation, mRNA processing and in stabilization of genomic DNA structure. PMID: 22057438
    31. This is the first study to identify liver FBPase as a previously unknown regulator of appetite and adiposity and describes a novel process by which the liver participates in body weight regulation. PMID: 22517657
    32. AMP binding pattern of the muscle isozyme of fructose-1,6-bisphosphatase is quite similar to that of the liver isozyme and the T conformations of the two isozymes are nearly the same PMID: 22120740
    33. epigenetic inactivation of FBP1 is also common in human liver and colon cancer. FBP1 appears to be a functional tumor suppressor involved in the liver and colon carcinogenesis PMID: 22039417
    34. Novel compound heterozygous mutations in the fructose-1,6-bisphosphatase gene cause hypoglycemia and lactic acidosis. PMID: 20096900
    35. first report on mutations in patients with FBP deficiency of Arab ethnicity,two novel mutations in the FBP1 gene encode for a duplication of two amino acids and a truncation in the FBP1 protein in these families. PMID: 19259699
    36. Data show that NF-kappaB functions downstream of Ras to promote epigenetic downregulation of FBP1. PMID: 19881551
    37. Data show that EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine. PMID: 19505453
    38. liver fructose-1,6-bisphosphatase coupled with phosphofructokinase (PFK) plays a crucial role in the metabolism of pancreatic islet cells PMID: 15965961
    39. Upregulation of FBPase in pancreatic islet beta-cells in states of lipid oversupply and type 2 diabetes, contributes to insulin secretory dysfunction. PMID: 18375435
    40. Human FBP1 was found to regulate mouse endogenous glucose production and whole body glucose homeostasis in a liver-specific transgenic model. PMID: 18780768
    41. this is the first experimental evidence confirming that the KKKGK motif can act as a functional NLS fructose 1,6-bisphosphatase . PMID: 19626708

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  • 相关疾病：
    Fructose-1,6-bisphosphatase deficiency (FBP1D)
  • 蛋白家族：
    FBPase class 1 family
  • 组织特异性：
    Expressed in pancreatic islets.
  • 数据库链接：

    HGNC: 3606

    OMIM: 229700

    KEGG: hsa:2203

    STRING: 9606.ENSP00000364475

    UniGene: Hs.494496